Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Epothilone B (10 nM, 18 hr; +), using Phospho-Histone H3 (Ser10) (D2C8) XP ® Rabbit mAb #3377 (upper) or Histone H3 (D1H2) XP ® Rabbit mAb #4499 (lower).
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Epothilone B (10 nM, 18 hr; +), using Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) (D13A11) XP ® Rabbit mAb #2914 (upper) or Aurora A (1F8) Mouse mAb #12100 and COX IV (3E11) Rabbit mAb #4850 (lower).
Chemical structure of epothilone B.