Thapsigargin
| Name | Thapsigargin |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 12758 |
| Prices | $119.00 |
| Sizes | 1 mg |
| Formula | C34H50O12 |
| Molecular Weight | 650.8 g/mol |
| Purity | >97% |
| Description | Thapsigargin is a cell-permeable sesquiterpene lactone derived from the plant Thapsia garganica that acts as a tumor promoter in mammalian cells (1,2). Studies show that thapsagargin causes a rapid increase in cytosolic Ca 2+ concentrations via discharge of intracellular Ca 2+ stores. Research indicates that this increase in cytosolic calcium results from the specific inhibition of endoplasmic reticulum Ca 2+ -ATPases (IC 50 = ~30 nM), and does not involve the hydrolysis of inositol phospholipids or protein kinase C (1,2). This disruption of calcium homeostasis is widely used in research studies to induce ER stress. Conflicting information regarding the role of thapsigargin in autophagy has been reported, but recent evidence points to thapsigargin inhibiting autophagy by blocking autophagosome fusion with lysosomes (3-5). |
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Product images
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Thapsigargin (2 nM, 16 hr; +), using OS-9 (D8P4G) Rabbit mAb #12497 (upper) and GAPDH (D16H11) XP ® Rabbit mAb #5174 (lower).
Western blot analysis of extracts from C2C12 cells, untreated (-) or treated with Thapsigargin (300 nM, 30 min; +), using Phospho-eIF2α (Ser51) (D9G8) XP ® Rabbit mAb #3398 (upper) and eIF2α Antibody #9722 (lower).
Chemical structure of Thapsigargin.