| Description |
This N-terminal HA-tagged protein is a potent, irreversible and specific inhibitor of ATG-specific isopeptidases (such as ATG4B, Cat # E-400). ATG4B activities include the processing of ATG8 precursor proteins and the removal of ATG8 proteins that are conjugated to phosphatidylethanolamine during autophagy. These processes can be inhibited by this vinyl sulfone derivative which reacts with the ATG4B active site cysteine. The HA peptide sequence (YPYDVPDYA) is derived from the influenza hemagglutinin protein. This epitope allows for the sensitive identification or purification of such deconjugating activities since it is specifically recognized by anti-HA antibodies and/or anti-HA-agarose.
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