| Description |
Ubiquitin-Propargylamide (Ub-PA) is a potent, irreversible and specific inhibitor of C-terminal hydrolases (UCHs), ubiquitin-specific proteases (USPs), and other deubiquitinases including the Otubains (OTU’s). When mixed together, the reactive site cysteine of deubiquitinases and the C-terminal alkyne functional group of Ub-PA rapidly form a covalent bond (vinyl thioether) that is resistant to denaturing conditions. This product is useful for inhibiting the hydrolysis of poly-ubiquitin chains on substrate proteins in vitro and thus enhances poly-ubiquitin chain accumulation.
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