DFHBI 1T

• Supplier: Tocris Bioscience
• Catalog: 5609
• Prices: $235.00
• Sizes: 10 mg
• Cas Number: 1539318-36-9
• Chemical Name: (5Z)-5-[(3,5-Difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2-methyl-3-(2,2,2-trifluoroethyl)-4H-imidazol-4-one
• Formula: C13H9F5N2O2
• Molecular Weight: 320.21
• Purity: >98 %
• Description: Mimic of green fluorescent protein (GFP) fluorophore for imaging RNA in living cells. Fluorescence activated by binding to Spinach2 or Broccoli aptamers. Exhibits peak excitation maxima of 482 nm and peak fluorescence emission of 505 nm when bound to Spinach2, enabling imaging with GFP filter cubes. Exhibits higher specific fluorescence and lower background fluorescence when bound to Spinach2 compared with Spinach2-DFHBI.

Product References

Plug-and-play fluorophores extend the spectral properties of Spinach. - Plug-and-play fluorophores extend the spectral properties of Spinach.

Song W, Strack RL, Svensen N, Jaffrey SR. J Am Chem Soc. 2014 Jan 29;136(4):1198-201.

In-gel imaging of RNA processing using broccoli reveals optimal aptamer - In-gel imaging of RNA processing using broccoli reveals optimal aptamer

Filonov GS, Kam CW, Song W, Jaffrey SR. Chem Biol. 2015 May 21;22(5):649-60.