Dynamin Inhibitors: Dynole™ Series Kit
| Name | Dynamin Inhibitors: Dynole™ Series Kit |
|---|---|
| Supplier | Abcam |
| Catalog | ab120474 |
| Description | DynoleTM chemical series collectionConvenient kit containing dynamin inhibitor Dynole-34-2™ (ab120463; 1 mg) and negative control Dynole-31-2™ (ab120464; 1mg) from the Dynole chemical series. Dynole-34-2™ targets dynamin at the GTPase Allosteric Site (GAS) domain and inhibits dynamin and endocytosis in enzymatic and cell based assays. The inhibitor and negative control are based on the same chemical scaffold.TargetDescriptionGTPase Allosteric SiteDynole-34-2™: Potent dynamin I and dynamin II inhibitorGTPase Allosteric SiteDynole-31-2™: Negative control for Dynole 34-2™ Convenient kit containing dynamin inhibitor Dynole-34-2™ ab120464; 1mg) from the Dynole chemical series. Dynole-34-2™ targets dynamin at the GTPase Allosteric Site (GAS) domain and inhibits dynamin and endocytosis in enzymatic and cell based assays. The inhibitor and negative control are based on the same chemical scaffold. |
| Supplier Page | Shop |
Product images
ab66705 staining PAI1 in HeLa cells treated with dynole-31-2™ (ab120464), by ICC/IF. No change in PAI1 expression with increased concentration of dynole-31-2™ (negative control for dynole 34-2™ (ab120463), as described in literature.The cells were incubated at 37°C for 6h in media containing different concentrations of ab120464 (dynole-31-2™) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab66705 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
ab66705 staining PAI1 in HeLa cells treated with dynole-34-2™; (ab120463), by ICC/IF. Increase in PAI1 expression correlates with increased concentration of dynole-34-2™, as described in literature.The cells were incubated at 37°C for 24h in media containing different concentrations of ab120463 (dynole-34-2™) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab66705 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Product References
Lipid droplet breakdown requires dynamin 2 for vesiculation of autolysosomal - Lipid droplet breakdown requires dynamin 2 for vesiculation of autolysosomal
Schulze RJ, Weller SG, Schroeder B, Krueger EW, Chi S, Casey CA, McNiven MA. J Cell Biol. 2013 Oct 28;203(2):315-26.
Inhibition of dynamin mediated endocytosis by the dynoles--synthesis and - Inhibition of dynamin mediated endocytosis by the dynoles--synthesis and
Hill TA, Gordon CP, McGeachie AB, Venn-Brown B, Odell LR, Chau N, Quan A, Mariana A, Sakoff JA, Chircop M, Robinson PJ, McCluskey A. J Med Chem. 2009 Jun 25;52(12):3762-73.