Dynole® 31-2

• Supplier: Abcam
• Catalog: ab120464
• Chemical Name: 2-Cyano-N-propyl-3-[1-(3-dimethylaminopropyl)-1H-indol-3-yl]acrylamide
• Formula: C20H26N4O
• Molecular Weight: 338.45
• Purity: > 98%
• Description: Negative control for Dynole® 34-2

Product images

ab66705 staining PAI1 in HeLa cells treated with dynole-31-2™ (ab120464), by ICC/IF. No change in PAI1 expression with increased concentration of dynole-31-2™ (negative control for dynole 34-2™ (ab120463), as described in literature.The cells were incubated at 37°C for 6h in media containing different concentrations of ab120464 (dynole-31-2™) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab66705 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

Product References

Host cell entry of respiratory syncytial virus involves macropinocytosis followed - Host cell entry of respiratory syncytial virus involves macropinocytosis followed

Krzyzaniak MA, Zumstein MT, Gerez JA, Picotti P, Helenius A. PLoS Pathog. 2013;9(4):e1003309.

Intracellular curvature-generating proteins in cell-to-cell fusion. - Intracellular curvature-generating proteins in cell-to-cell fusion.

Richard JP, Leikina E, Langen R, Henne WM, Popova M, Balla T, McMahon HT, Kozlov MM, Chernomordik LV. Biochem J. 2011 Dec 1;440(2):185-93.

Inhibition of HIV-1 endocytosis allows lipid mixing at the plasma membrane, but - Inhibition of HIV-1 endocytosis allows lipid mixing at the plasma membrane, but

de la Vega M, Marin M, Kondo N, Miyauchi K, Kim Y, Epand RF, Epand RM, Melikyan GB. Retrovirology. 2011 Dec 6;8:99.