ab51110 staining AMPKα 1 + AMPKα 2 (phosphoT172) in HeLa cells treated with flufenamic acid (ab120354), by ICC/IF. Increase in AMPKα 1 + AMPKα 2 (phosphoT172) nuclear expression correlates with increased concentration of flufenamic acid, as described in literature.The cells were incubated at 37°C for 30 minutes in media containing different concentrations of ab120354 (flufenamic acid) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab51110 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.