ab46154 staining VEGF in MCF7 cells treated with HA 1077 (ab120306), by ICC/IF. Decrease in VEGF expression correlates with increased concentration of HA 1077 as described in literature.The cells were incubated at 37°C for 1h in media containing different concentrations of ab120306 (HA 1077) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab46154 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.