Ionomycin Ca2+ Salt
| Name | Ionomycin Ca2+ Salt |
|---|---|
| Supplier | Abcam |
| Catalog | ab120116 |
| Cas Number | 56092-82-1 |
| Pubchem | 6446270 |
| Chemical Name | (4R,6S,8S,10Z,12R,14R,16E,18R,19R,20S,21S)-11,19,21-Trihydroxy-4,6,8,12,14,18,20-heptamethyl-22-[(2S,2'R,5S,5'S)-octahydro-5'-[(1R)-1-hydroxyethyl]-2,5'-dimethyl[2,2'-bifuran]-5-yl]-9-oxo-10,16-docosadienoic acid calcium salt |
| Formula | C41H70CaO9 |
| Molecular Weight | 747.08 |
| Purity | > 99% |
| Description | Ca2+ ionophore |
| Supplier Page | Shop |
Product images
ab58668 staining ATF3 in A549 cells treated with ionomycin Ca2+ salt (ab120116), by ICC/IF. Increase in ATF3 expression correlates with increased concentration of ionomycin Ca2+ salt, as described in literature.The cells were incubated at 37°C for 2h in media containing different concentrations of ab120116 (ionomycin Ca2+ salt) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab58668 (10 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Sandwich ELISA - IFN gamma Human ELISA Kit (ab46025)Jurkat were stimulated for 48 hours with 50 ng x mL-1 of PMA (ab120297) and 1 uM Ionomycin (ab120116) and PBMCs were stimulated for 48 hours with 2 % PHA-M (LifeTechnologies). Cell free supernatants were tested, showing results after background signal was subtracted (duplicates +/- SD).
Product References
Oxytocin-stimulated NFAT transcriptional activation in human myometrial cells. - Oxytocin-stimulated NFAT transcriptional activation in human myometrial cells.
Pont JN, McArdle CA, Lopez Bernal A. Mol Endocrinol. 2012 Oct;26(10):1743-56. Epub 2012 Aug 17.
PDGF enhances store-operated Ca2+ entry by upregulating STIM1/Orai1 via - PDGF enhances store-operated Ca2+ entry by upregulating STIM1/Orai1 via
Ogawa A, Firth AL, Smith KA, Maliakal MV, Yuan JX. Am J Physiol Cell Physiol. 2012 Jan 15;302(2):C405-11. doi: