KU-55933

• Supplier: Abcam
• Catalog: ab120637
• Cas Number: 587871-26-9
• Chemical Name: 2-(4-Morpholinyl)-6-(1-thianthrenyl)-4H-pyran-4-one
• Formula: C21H17NO3S2
• Molecular Weight: 395.49
• Purity: > 99%
• Description: Potent, selective, competitive ATM kinase inhibitor

Product images

HepG2 cells were incubated at 37°C for 60 minutes with vehicle control (0 µM) and different concentrations of KU-55933 (ab120637). Decreased expression of Acetyl Coenzyme A Carboxylase (phospho S79) (ab31931) in HepG2 cells correlates with an increase in KU-55933 concentration, as described in literature.Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab31931 at 1 µg /ml and ab8227 at 1 µg /ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.

Product References

Activation of the prereplication complex is blocked by mimosine through reactive - Activation of the prereplication complex is blocked by mimosine through reactive

Kubota S, Fukumoto Y, Ishibashi K, Soeda S, Kubota S, Yuki R, Nakayama Y, Aoyama K, Yamaguchi N, Yamaguchi N. J Biol Chem. 2014 Feb 28;289(9):5730-46.

DNA damage-specific deubiquitination regulates Rad18 functions to suppress - DNA damage-specific deubiquitination regulates Rad18 functions to suppress

Zeman MK, Lin JR, Freire R, Cimprich KA. J Cell Biol. 2014 Jul 21;206(2):183-97.