Leptomycin B (ethanol solution)
| Name | Leptomycin B (ethanol solution) |
|---|---|
| Supplier | Abcam |
| Catalog | ab120501 |
| Cas Number | 87081-35-4 |
| Pubchem | 6917907 |
| Chemical Name | (2E,5S,6R,7S,9R,10E,12E,15R,16Z,18E)-19-[(2S,3S)-3,6-Dihydro-3-methyl-6-oxo-2H-pyran-2-yl]-17-ethyl-6-hydroxy-3,5,7,9,11,15-hexamethyl-8-oxo-2,10,12,16,18-nonadecapentaenoic acid |
| Formula | C33H48O6 |
| Molecular Weight | 540.74 |
| Purity | > 98% |
| Description | Antibiotic, antifungal. Inhibitor of CRM-1 mediated nuclear export. |
| Supplier Page | Shop |
Product images
ab38335 staining p75 in HeLa cells treated with Leptomycin B (ethanol solution) (ab120501), by ICC/IF. Increase in p75 expression in the nucleous and cytoplasm correlates with increased concentration of Leptomycin B (ethanol solution), as described in literature.The cells were incubated at 37°C for 2h in media containing different concentrations of ab120501 (Leptomycin B (ethanol solution)) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab38335 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.