ab113202 staining NET1 in PC-12 cells treated with milnacipran hydrochloride (ab120755), by ICC/IF. Decrease of NET1 expression correlates with increased concentration of milnacipran hydrochloride, as described in literature.The NGF treated cells were incubated at 37°C for 6 hour in media containing different concentrations of ab120755 (milnacipran hydrochloride ) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab113202 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.