ab80579 staining tau in SKNSH cells treated with prostaglandin J2 (ab120913), by ICC/IF. Expression of tau expression is restringed to the perinuclear zone with increased concentration of prostaglandin J2, as described in literature.The cells were incubated at 37°C for 6 hours in media containing different concentrations of ab120913 (prostaglandin J2) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab80579 (10 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei (blue) were counterstained with DAPI and membrane is was stained using WGA (red).