ab51031 staining beclin1 in MCF7 cells treated with pterostilbene (ab120961), by ICC/IF. Increase in beclin1 expression correlates with increased concentration of pterostilbene as described in literature.The cells were incubated at 37°C for 48h in media containing different concentrations of ab120961 (pterostilbene) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab51031 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.