ab118171 ligand binding and displacement. Top (A, C, E): ab118171 ligand (100 nM) binding to three different live cell lines expressing β1 (A), β2 (C) and β3 (E) adrenoceptors. Bottom (B, D, F): Binding blocked in the same three cell lines using the unlabelled competitor ICI118551 at 1 µM (β1: B, β2: D) or SR59230A at 1 µM (β3: F). Nuclei have been counter-stained with Hoechst.
The apparent KD was calculated from the rightward shift of the agonist response curve in the presence of ab118171, compared to the response curve for the agonist alone, for β1 receptor expressing cell line.
The apparent KD was calculated from the rightward shift of the agonist response curve in the presence of ab118171, compared to the response curve for the agonist alone, for β2 receptor expressing cell line.
The apparent KD was calculated from the rightward shift of the agonist response curve in the presence of ab118171, compared to the response curve for the agonist alone, for β3 receptor expressing cell line.
EC50 value weak partial agonism <10 % Max isoprenaline-stimulated.
EC50 value 7.09 = 90% Max isoprenaline-stimulated