Staurosporine
| Name | Staurosporine |
|---|---|
| Supplier | Abcam |
| Catalog | ab120056 |
| Cas Number | 62996-74-1 |
| Pubchem | 44259 |
| Chemical Name | [9S-(9α,10β,11β,13α)]-2,3,10,11,12,13-Hexahydro-10-methoxy-9-methyl-11-(methylamino)-9,13-epoxy-1H,9H-diindolo[1,2,3-gh:3,2,1-lm]pyrrolo[3,4-j][1,7]benzodiazonin-1-one |
| Formula | C28H26N4O3 |
| Molecular Weight | 466.54 |
| Purity | > 98% |
| Description | Protein kinase inhibitor |
| Supplier Page | Shop |
Product images
Lane 1: HeLa, vehicle (DMSO) treated for 4 hours Lane 2: HeLa 1 µM staurpsorine (ab120056), 4 hours Load 20 µg/lane 5% milk/PBST for block and antibody diluent Primary antibodies (2 hours, room temp) All lanes: ab136812 250X Primary Antibodies Cocktail, 1/250 dilution Secondary antibodies (1 hour, room temp) All lanes: ab136812 100X HRP-Conjugated Secondary Antibodies Cocktail, 1/100 dilution
Example of HeLa staurosporine (ab120056) treated cell lysate titration. Background-subtracted data values (mean +/- SD) are graphed.
HeLa and Jurkat cells were treated with 1 µM Staurosporine (STS) (ab120056) for 4 hours in complete cell culture media to induce apoptosis and cleaved PARP protein. Untreated and STS treated HeLa and Jurkat lysates were prepared in 1X Cell Extraction Buffer PTR and tested the Cleaved PARP SimpleStepTM ELISA. Raw OD 450 nm values are shown for 500 µg/mL lysate loads.
Example of IC50 determination. HeLa cells were treated with a dose titration of Staurosporine for 4 hours in complete media. Cells were cultured and treated in a 96-well cell culture microtiter plate. Lysates were prepared by direct in-well lysis without media removal: 2X Cell Extraction Buffer PTR was added to an equal volume of media and then resulting lysate was used directly in the Cleaved PARP SimpleStepTM ELISA assay. Raw values for triplicate measurements are plotted. The calculated IC50 is 0.77 µM.
Demonstration of Cleaved PARP capture antibody specificity by western blot assay. 20 µg of HeLa extracts that were untreated or treated for 4 hours with 1 µM Staurosporine were analyzed by western blot. The GAPDH blot is included to show the relative loads of each lysate. In the HeLa cell line, Staurosporine treatment is required to detect cleaved PARP protein, as observed in the SimpleStep ELISA.
Product References
BRD4 is an atypical kinase that phosphorylates serine2 of the RNA polymerase II - BRD4 is an atypical kinase that phosphorylates serine2 of the RNA polymerase II
Devaiah BN, Lewis BA, Cherman N, Hewitt MC, Albrecht BK, Robey PG, Ozato K, Sims RJ 3rd, Singer DS. Proc Natl Acad Sci U S A. 2012 May 1;109(18):6927-32. doi:
Mechanism of a genetically encoded dark-to-bright reporter for caspase activity. - Mechanism of a genetically encoded dark-to-bright reporter for caspase activity.
Nicholls SB, Chu J, Abbruzzese G, Tremblay KD, Hardy JA. J Biol Chem. 2011 Jul 15;286(28):24977-86.