Anandamide (ethanol solution)
| Name | Anandamide (ethanol solution) |
|---|---|
| Supplier | Abcam |
| Catalog | ab120087 |
| Cas Number | 94421-68-8 |
| Pubchem | 5281969 |
| Chemical Name | (5Z,8Z,11Z,14Z)-N-(2-Hydroxyethyl)icosa-5,8,11,14-tetraenamide |
| Formula | C22H37NO2 |
| Molecular Weight | 347.54 |
| Purity | > 98% |
| Description | Endogenous cannabinoid |
| Supplier Page | Shop |
Product images
ab81298 staining FAK (phospho Y397) in SK-N-SH cells treated with anandamide (ethanol solution) (ab120087), by ICC/IF. Increase in FAK (phospho Y397) expression correlates with increased concentration of anandamide (ethanol solution), as described in literature.The cells were incubated at 37°C for 10 minutes in media containing different concentrations of ab120087 (anandamide (ethanol solution)) in ethanol, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab81298 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue. Membranes are stained in red with WGA.
Product References
Docosahexaenoyl ethanolamide improves glucose uptake and alters endocannabinoid - Docosahexaenoyl ethanolamide improves glucose uptake and alters endocannabinoid
Kim J, Carlson ME, Watkins BA. Front Physiol. 2014 Mar 21;5:100.
Characterization of the endocannabinoid system, CB(1) receptor signalling and - Characterization of the endocannabinoid system, CB(1) receptor signalling and
Brighton PJ, Marczylo TH, Rana S, Konje JC, Willets JM. Br J Pharmacol. 2011 Nov;164(5):1479-94.