| Description |
Porcine Primary Vein Smooth Muscle Cells from Cell Biologics are isolated from inferior vena cava tissue of Porcine. Porcine Primary Vein Smooth Muscle Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Cell Biologics Culture Complete Growth Medium generally for 3-7 days. Prior to shipping, cells at passage 1 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6 cells per ml and is delivered frozen. The cells are characterized by immunofluorescent method with antibodies to α-smooth muscle actin. Porcine Primary Vein Smooth Muscle Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Cell Biologics (Cells may be expanded for 2 or 3 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics). Repeated freezing and thawing of cells is not recommended.
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