RC3H1

Gene Symbol RC3H1
Entrez Gene 149041
Alt Symbol RNF198, ROQUIN
Species Human
Gene Type protein-coding
Description ring finger and CCCH-type domains 1
Other Description RING finger and C3H zinc finger protein 1|RING finger and CCCH-type zinc finger domain-containing protein 1|RING finger protein 198|probable E3 ubiquitin-protein ligase Roquin|roquin-1
Swissprots Q5W180 Q5W181 Q5TC82 B3KVK1 Q8N9V1 Q8IVE6
Accessions EAW90972 Q5TC82 AB095945 BAC23121 AK093501 BAC04186 AK122948 BAG53813 AY726570 BC136784 AAI36785 BC144408 AAI44409 BX537597 XM_005244921 XP_005244978 XM_011509230 XP_011507532 XM_011509231 XP_011507533 NM_001300850 NP_001287779 NM_001300851 NP_001287780 NM_001300852 NP_001287781 NM_172071 NP_742068
Function Post-transcriptional repressor of mRNAs containing a conserved stem loop motif, called constitutive decay element (CDE), which is often located in the 3'-UTR, as in HMGXB3, ICOS, IER3, NFKBID, NFKBIZ, PPP1R10, TNF and in many more mRNAs (By similarity). Binds to CDE and promotes mRNA deadenylation and degradation. This process does not involve miRNAs (By similarity). In follicular helper T (Tfh) cells, represses of ICOS and TNFRSF4 expression, thus preventing spontaneous Tfh cell differentiation, germinal center B-cell differentiation in the absence of immunization and autoimmunity (By similarity). In resting or LPS- stimulated macrophages, controls inflammation by suppressing TNF expression (By similarity). Also recognizes CDE in its own mRNA and in that of paralogous RC3H2, possibly leading to feedback loop regulation (By similarity). {ECO:0000250}.
Subcellular Location Cytoplasm, P-body {ECO:0000250}. Note=During stress, such as that induced by arsenite, localizes to cytosolic stress granules. {ECO:0000250}.
Tissue Specificity Widely expressed. Expressed at higher level in cerebellum, spleen, ovary and liver. {ECO:0000269|Ref.3}.

Roquin (T-14) - sc-162123 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-RC3H1 antibody - ab70195 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-RC3H1 antibody (ab70195) at 1 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgLane 4 : 293T whole cell lysate at 50 µg

Anti-RC3H1 antibody - ab156272 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-RC3H1 antibody (ab156272) at 1 µg/ml + HeLa cell lysate at 15 µg

Anti-RC3H1 antibody - ab70196 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-RC3H1 antibody (ab70196) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgLane 4 : 293T whole cell lysate at 50 µg

Anti-RC3H1 antibody - ab116291 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-RC3H1 antibody (ab116291) at 1/100 dilution + NCI-H460 cell line lysates at 35 µg

Anti-RC3H1 / ROQUIN Antibody (aa500-550) - LS-C93370 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-RC3H1 / ROQUIN Antibody (C-Terminus) - LS-C93371 from LifeSpan Bioscience

Prices $545.00
Sizes 20 µg
Host Rabbit
Clonality Polyclonal

Anti-RC3H1 / ROQUIN Antibody (aa1015-1043, Biotin) - LS-C239863 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RC3H1 / ROQUIN Antibody (aa1015-1043, AP) - LS-C239864 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RC3H1 / ROQUIN Antibody (aa1015-1043, APC) - LS-C239862 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RC3H1 / ROQUIN Antibody (aa1015-1043, FITC) - LS-C239865 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RC3H1 / ROQUIN Antibody (aa1015-1043, PE) - LS-C239867 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RC3H1 / ROQUIN Antibody (aa1015-1043, Azide-free, HRP) - LS-C248636 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RC3H1 / ROQUIN Antibody (C-Terminus) - LS-C287399 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-RC3H1 / ROQUIN Antibody (aa1014-1043) - LS-C324584 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RC3H1 / ROQUIN Antibody (aa500-550) - LS-C287398 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Mouse Roquin by Western Blot. Samples: Whole cell lysate (50 ug) from TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-Roquin antibody used for WB at 2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Anti-RC3H1 / ROQUIN Antibody (aa1015-1043) - LS-C162510 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
RC3H1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human kidney tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Anti-RC3H1 / ROQUIN Antibody (C-Terminus) - LS-C201054 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry of RC3H1 in human small intestine tissue with RC3H1 antibody at 5 ug/ml.

Roquin Antibody - NB100-655 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: Roquin Antibody [NB100-655] - Detection of human Roquin on HeLa whole cell lysate using NB100-655. Roquin was also immunoprecipitated using NB100-656 at 3 ug/mg lysate.

Roquin Antibody - NBP1-89591 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: Roquin Antibody [NBP1-89591] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane, cytosol & the Golgi apparatus.

Roquin Antibody - NBP1-89589 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Roquin Antibody - NB100-656 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: Roquin Antibody [NB100-656] - Samples: A) Whole cell lysate from HeLa (5, 15 and 50 ug) and 293T (T, 50 ug) cells. B) Whole cell lysate (1 mg/IP; 1/4 of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Roquin antibody NB100-656 used at 0.04 ug/ml for WB (A and B) and at 3 ug/mg lysate for IP (B). Roquin was also immunoprecipitated using rabbit anti-Roquin antibody NB100-655 at 3 ug/mg lysate. Detection: Chemiluminescence with an exposure time of 30 seconds.

Roquin Antibody - NBP1-89590 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-RC3H1 antibody produced in rabbit - HPA027428 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-RC3H1: <B>Cat. No. HPA027428</B>: Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in subsets of islet cells.

Anti-RC3H1 antibody produced in rabbit - HPA027448 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-RC3H1: <B>Cat. No. HPA027448</B>: Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
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