Gene Symbol | INSIG1 |
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Entrez Gene | 3638 |
Alt Symbol | CL-6, CL6 |
Species | Human |
Gene Type | protein-coding |
Description | insulin induced gene 1 |
Other Description | INSIG-1 membrane protein|insulin-induced gene 1 protein |
Swissprots | Q53XW8 A4D2N1 A8K6L0 Q9BUV5 O15503 |
Accessions | AAB69121 EAL23728 EAL23729 EAL23730 EAX04529 EAX04530 EAX04531 EAX04532 O15503 AA565248 AF086365 AK095977 AK291675 BAF84364 AL541939 AY112745 AAM44086 BC001880 AAH01880 BI550548 BI918342 BM995866 BQ059075 BT007227 AAP35891 DA590854 DA738378 DN996424 XM_005249542 XP_005249599 XM_005249543 XP_005249600 NM_005542 NP_005533 NM_198336 NP_938150 NM_198337 NP_938151 |
Function | Mediates feedback control of cholesterol synthesis by controlling SCAP and HMGCR. Functions by blocking the processing of sterol regulatory element-binding proteins (SREBPs). Capable of retaining the SCAP-SREBF2 complex in the ER thus preventing it from escorting SREBPs to the Golgi. Initiates the sterol-mediated ubiquitin-mediated endoplasmic reticulum-associated degradation (ERAD) of HMGCR via recruitment of the reductase to the ubiquitin ligase, AMFR/gp78. May play a role in growth and differentiation of tissues involved in metabolic control. May play a regulatory role during G0/G1 transition of cell growth. {ECO:0000269|PubMed:12202038, ECO:0000269|PubMed:12535518, ECO:0000269|PubMed:16168377, ECO:0000269|PubMed:16399501, ECO:0000269|PubMed:16606821}. |
Subcellular Location | Endoplasmic reticulum membrane {ECO:0000269|PubMed:12202038}; Multi-pass membrane protein {ECO:0000269|PubMed:12202038}. |
Tissue Specificity | Expressed in all tissues tested with highest expression in the liver. {ECO:0000269|PubMed:12202038}. |
INSIG-1 CRISPR/Cas9 KO Plasmid (h) - sc-401381 from Santa Cruz Biotechnology
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INSIG-1 HDR Plasmid (h) - sc-401381-HDR from Santa Cruz Biotechnology
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INSIG-1 Double Nickase Plasmid (h) - sc-401381-NIC from Santa Cruz Biotechnology
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INSIG-1 Double Nickase Plasmid (h2) - sc-401381-NIC-2 from Santa Cruz Biotechnology
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INSIG-1 CRISPR Activation Plasmid (h) - sc-401381-ACT from Santa Cruz Biotechnology
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INSIG-1 CRISPR Activation Plasmid (h2) - sc-401381-ACT-2 from Santa Cruz Biotechnology
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INSIG-1 Lentiviral Activation Particles (h) - sc-401381-LAC from Santa Cruz Biotechnology
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INSIG-1 Lentiviral Activation Particles (h2) - sc-401381-LAC-2 from Santa Cruz Biotechnology
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INSIG-1 siRNA (h) - sc-44432 from Santa Cruz Biotechnology
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INSIG-1 shRNA Plasmid (h) - sc-44432-SH from Santa Cruz Biotechnology
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INSIG-1 shRNA (h) Lentiviral Particles - sc-44432-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU086971 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN10699 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND10699 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR10985 from Sigma-Aldrich
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INSIG1 siRNA (Human) - CRH2439 from Cohesion Biosciences
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INSIG1 - MBS8219080 from MyBioSource
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shRNA set against Human INSIG1 (NM_005542.4) - SHH319821 from Creative biogene
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Human INSIG1 siRNA - orb262941 from Biorbyt
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INSIG1 shRNA Plasmid (Human) - CHH2439 from Cohesion Biosciences
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INSIG1 miRNA 3'UTR clone - MiUTR3H-04633 from Creative biogene
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INSIG1 miRNA 3'UTR clone - MiUTR3H-04634 from Creative biogene
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