| Gene Symbol | Alox15 |
|---|---|
| Entrez Gene | 11687 |
| Alt Symbol | 12-LO, 12/15-LO, 15-LOX, Alox12l, L-12LO |
| Species | Mouse |
| Gene Type | protein-coding |
| Description | arachidonate 15-lipoxygenase |
| Other Description | 12-LOX|12/15-lipoxygenase|arachidonate 12-lipoxygenase, leukocyte-type|arachidonate omega-6 lipoxygenase |
| Swissprots | Q4FJY9 P39654 Q5F2E3 Q6PHB2 |
| Accessions | ABV02575 EDL12554 P39654 AK142371 BAE25045 BC056625 AAH56625 BC081546 AAH81546 CT010263 CAJ18471 L34570 AAA64930 U04331 AAA20658 XM_006532036 XP_006532099 XM_006537114 XP_006537177 NM_009660 NP_033790 |
| Function | Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Converts arachidonic acid into 12- hydroperoxyeicosatetraenoic acid/12-HPETE and 15- hydroperoxyeicosatetraenoic acid/15-HPETE. Also converts linoleic acid to 13-hydroperoxyoctadecadienoic acid. May also act on (12S)- hydroperoxyeicosatetraenoic acid/(12S)-HPETE to produce hepoxilin A3. Probably plays an important role in the immune and inflammatory responses. Through the oxygenation of membrane-bound phosphatidylethanolamine in macrophages may favor clearance of apoptotic cells during inflammation by resident macrophages and prevent an autoimmune response associated with the clearance of apoptotic cells by inflammatory monocytes. In parallel, may regulate actin polymerization which is crucial for several biological processes, including macrophage function. May also regulate macrophage function throug |
| Subcellular Location | Cytoplasm, cytosol {ECO:0000269|PubMed:11278875}. Cell membrane {ECO:0000269|PubMed:11278875}; Peripheral membrane protein {ECO:0000269|PubMed:11278875}. Lipid droplet {ECO:0000250}. Note=Predominantly cytosolic; becomes enriched at membranes upon calcium binding. Translocates from the cytosol to the plasma membrane when stimulated by IL13/interleukin-13 and in macrophages binding apoptotic cells. |
| Tissue Specificity | Found in pituitary and pineal glands as well as leukocytes, kidney, aorta, small intestine and cornea. Also expressed by resident peritoneal macrophages (at protein level). {ECO:0000269|PubMed:15708862, ECO:0000269|PubMed:22503541, ECO:0000269|PubMed:8188678, ECO:0000269|PubMed:8798642}. |
| Top Pathways | Arachidonic acid metabolism, Linoleic acid metabolism |
Leukocyte-type 12-LO CRISPR/Cas9 KO Plasmid (m) - sc-419089 from Santa Cruz Biotechnology
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Leukocyte-type 12-LO HDR Plasmid (m) - sc-419089-HDR from Santa Cruz Biotechnology
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Leukocyte-type 12-LO Double Nickase Plasmid (m) - sc-419089-NIC from Santa Cruz Biotechnology
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Leukocyte-type 12-LO Double Nickase Plasmid (m2) - sc-419089-NIC-2 from Santa Cruz Biotechnology
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Leukocyte-type 12-LO CRISPR Activation Plasmid (m) - sc-419089-ACT from Santa Cruz Biotechnology
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Leukocyte-type 12-LO CRISPR Activation Plasmid (m2) - sc-419089-ACT-2 from Santa Cruz Biotechnology
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Leukocyte-type 12-LO Lentiviral Activation Particles (m) - sc-419089-LAC from Santa Cruz Biotechnology
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Leukocyte-type 12-LO Lentiviral Activation Particles (m2) - sc-419089-LAC-2 from Santa Cruz Biotechnology
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Leukocyte-type 12-LO siRNA (m) - sc-146714 from Santa Cruz Biotechnology
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Leukocyte-type 12-LO shRNA Plasmid (m) - sc-146714-SH from Santa Cruz Biotechnology
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Leukocyte-type 12-LO shRNA (m) Lentiviral Particles - sc-146714-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EMU071621 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN26591 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND26591 from Sigma-Aldrich
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ALOX15 siRNA (Mouse) - CRM0147 from Cohesion Biosciences
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ALOX15 - MBS8215137 from MyBioSource
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shRNA set against Mouse ALOX15 (NM_009660.3) - SHH235370 from Creative biogene
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shRNA set against Mouse Alox15(NM_009660.3) - SHG032011 from Creative biogene
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Leukocyte-type 12-LO CRISPR/Cas9 KO Plasmid (m2) - sc-419089-KO-2 from Santa Cruz Biotechnology
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Leukocyte-type 12-LO HDR Plasmid (m2) - sc-419089-HDR-2 from Santa Cruz Biotechnology
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Mouse ALOX15 siRNA - orb279623 from Biorbyt
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ALOX15 shRNA Plasmid (Mouse) - CHM0147 from Cohesion Biosciences
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ALOX15 miRNA 3'UTR clone - MiUTR1M-01420 from Creative biogene
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