LIN28B
| Gene Symbol | LIN28B |
|---|---|
| Entrez Gene | 389421 |
| Alt Symbol | CSDD2 |
| Species | Human |
| Gene Type | protein-coding |
| Description | lin-28 homolog B (C. elegans) |
| Other Description | Lin-28.2|lin-28B|protein lin-28 homolog B |
| Swissprots | B2RPN6 A1L165 Q6ZN17 Q5TCM4 |
| Accessions | CDI44686 EAW48433 EAW48434 Q6ZN17 AK131411 BAD18558 AW674597 BC127712 AAI27713 BC127713 AAI27714 BC137526 AAI37527 BC137527 AAI37528 BC141959 BC141960 AAI41961 BC150537 AAI50538 BC150538 AAI50539 DQ127228 AAZ38897 XM_006715477 XP_006715540 XM_011535818 XP_011534120 NM_001004317 NP_001004317 |
| Function | Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre- let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth. {ECO:0000269|PubMed:16971064, ECO:0000269|PubMed:18951094, ECO:0000269|PubMed:19703396}. |
| Subcellular Location | Cytoplasm. Nucleus. Note=Predominantly cytoplasmic at G1 phase, accumulates in the nucleus in S and G2 phases. The frequency of nuclear localization in S and in G2 phases is 60% and 30%, respectively. |
| Tissue Specificity | High expression in testis, fetal liver, placenta and in hepatocellular carcinoma (HCC). Isoform 1 is only detected in moderately and poorly differentiated HCC tissues and placenta (at protein level). Isoform 2 is detected in fetal liver, non-tumor liver tissues, as well as well-differentiated tumor tissues (at protein level). {ECO:0000269|PubMed:16971064}. |
LIN-28B CRISPR/Cas9 KO Plasmid (h) - sc-402851 from Santa Cruz Biotechnology
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LIN-28B HDR Plasmid (h) - sc-402851-HDR from Santa Cruz Biotechnology
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LIN-28B Double Nickase Plasmid (h) - sc-402851-NIC from Santa Cruz Biotechnology
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LIN-28B Double Nickase Plasmid (h2) - sc-402851-NIC-2 from Santa Cruz Biotechnology
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LIN-28B CRISPR Activation Plasmid (h) - sc-402851-ACT from Santa Cruz Biotechnology
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LIN-28B CRISPR Activation Plasmid (h2) - sc-402851-ACT-2 from Santa Cruz Biotechnology
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LIN-28B Lentiviral Activation Particles (h) - sc-402851-LAC from Santa Cruz Biotechnology
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LIN-28B Lentiviral Activation Particles (h2) - sc-402851-LAC-2 from Santa Cruz Biotechnology
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LIN-28B siRNA (h) - sc-105614 from Santa Cruz Biotechnology
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LIN-28B shRNA Plasmid (h) - sc-105614-SH from Santa Cruz Biotechnology
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LIN-28B shRNA (h) Lentiviral Particles - sc-105614-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU050781 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN11729 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND11729 from Sigma-Aldrich
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LIN28B siRNA (Human) - CRJ7883 from Cohesion Biosciences
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LIN-28B RNAi - H00389421-R01 from Novus Biologicals
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shRNA set against Human LIN28 (NM_001004317.3) - SHH330463 from Creative biogene
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Human LIN28B siRNA - orb278384 from Biorbyt
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LIN28B shRNA Plasmid (Human) - CHJ7883 from Cohesion Biosciences
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LIN28B miRNA 3'UTR clone - MiUTR4H-TG05075 from Creative biogene
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