RHOT2

Gene Symbol RHOT2
Entrez Gene 89941
Alt Symbol ARHT2, C16orf39, MIRO-2, MIRO2, RASL
Species Human
Gene Type protein-coding
Description ras homolog family member T2
Other Description mitochondrial Rho (MIRO) GTPase 2|mitochondrial Rho GTPase 2|ras homolog gene family, member T2
Swissprots Q9H7M8 A2IDC2 Q9BT60 Q8NF53 Q8IXI1 Q96C13 Q96S17
Accessions AAK61240 CBJ05785 CBJ21237 EAW85763 EAW85764 EAW85765 EAW85766 EAW85767 EAW85768 Q8IXI1 AJ517413 CAD56957 AK024450 BAB15740 AK090426 BAC03407 AK308984 AL834163 AY207375 AAP46090 BC004327 AAH04327 BC014942 AAH14942 DB465261 XM_005255660 XP_005255717 XM_005255661 XP_005255718 XM_005255662 XP_005255719 XM_005255663 XP_005255720 XM_005255664 XP_005255721 XM_006720970 XP_006721033 XM_006720971 XP_006721034 XM_006720973 XP_006721036 XR_932964 NM_138769 NP_620124
Function Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution (By similarity). {ECO:0000250}.
Subcellular Location Mitochondrion outer membrane {ECO:0000269|PubMed:12482879, ECO:0000269|PubMed:19528298}; Single-pass type IV membrane protein {ECO:0000269|PubMed:12482879, ECO:0000269|PubMed:19528298}. Note=Colocalizes with MGARP and RHOT2 at the mitochondria.
Tissue Specificity Ubiquitously expressed. Highly expressed in heart, liver, skeletal muscle, kidney and pancreas. {ECO:0000269|PubMed:12482879, ECO:0000269|PubMed:15218247}.

Miro2 Antibody - 14016 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using Miro2 Antibody.

Rho T2 (H-45) - sc-135387 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Rho T2 (H-45): sc-135387. Western blot analysis of Rho T2 expression in mouse testis tissue extract.

Rho T2 (T-13) - sc-165360 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Rho T2 (T-13): sc-165360. Western blot analysis of Rho T2 expression in mouse testis tissue extract.

Anti-RHOT2 antibody - ab154946 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab154946 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab154946 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Anti-RHOT2 antibody - ab172992 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-RHOT2 antibody (ab172992) at 1/100 dilution + HL60 cell lysate at 35 µg

Anti-RHOT2 antibody - ab67588 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-RHOT2 antibody (ab67588) at 1/500 dilutionLane 1 : RHOT2 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilutionObserved band size : 68 kDa (why is the actual band size different from the predicted?)

Anti-RHOT2 Antibody (aa41-90) - LS-C205836 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
RHOT2 antibody Western Blot of Lane 1: 20 ug untransfected HEK293T, Lane 2: 20ug RHOT2 transfected HEK293T. Primary Antibody dilution: 1:1000.

Anti-RHOT2 Antibody (aa291-320) - LS-C158849 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
RHOT2 Antibody western blot of HL-60 cell line lysates (35 ug/lane). The RHOT2 antibody detected the RHOT2 protein (arrow).

Anti-RHOT2 Antibody (aa291-320, APC) - LS-C268236 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RHOT2 Antibody (aa291-320, Biotin) - LS-C268237 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RHOT2 Antibody (aa291-320, FITC) - LS-C268238 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RHOT2 Antibody (aa291-320, Azide-free, HRP) - LS-C268239 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RHOT2 Antibody (aa291-320, PE) - LS-C268240 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RHOT2 Antibody (aa291-320, AP) - LS-C285053 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RHOT2 Antibody (aa290-320) - LS-C324670 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RHOT2 Antibody - LS-C341753 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

RHOT2 Antibody - NBP1-88982 from Novus Biologicals

Prices $129.00, $419.00
Sizes 25 µl, 100 µl
Host Rabbit
Clonality Polyclonal

RHOT2 Antibody - NBP1-88981 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

RHOT2 Antibody - H00089941-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: RHOT2 Antibody [H00089941-B01P] - Analysis of RHOT2 expression in transfected 293T cell line by RHOT2 polyclonal antibody. Lane 1: RHOT2 transfected lysate(67.98 KDa). Lane 2: Non-transfected lysate.

RHOT2 Antibody - H00089941-B01 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: RHOT2 Antibody [H00089941-B01] - analysis of RHOT2 expression in transfected 293T cell line by RHOT2 MaxPab polyclonal antibody (B01). Lane 1: RHOT2 transfected lysate (68.10 KDa). Lane 2: Non-transfected lysate.

RHOT2 Antibody - H00089941-A01 from Novus Biologicals

Prices $269.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: RHOT2 Antibody [H00089941-A01] - RHOT2 polyclonal antibody (A01), Lot # 050926JC01 Western Blot analysis of RHOT2 expression in MES-SA/Dx5 ( Cat # L021V1 ).

Anti-RHOT2 antibody produced in rabbit - HPA012624 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-RHOT2: <B>Cat. No. HPA012624</B>: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human skeletal muscle shows strong cytoplasmic positivity in myocytes.

Anti-RHOT2 antibody produced in mouse - SAB1408162 from Sigma-Aldrich

Prices $347.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-RHOT2: <B>Cat. No. SAB1408162</B>: Antibody concentration: 0.1-5 μg/mL. Specific band of ~68 kDa using RHOT2 transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.

Anti-RHOT2 antibody produced in rabbit - HPA012895 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-RHOT2: <B>Cat. No. HPA012895</B>: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human liver shows strong cytoplasmic positivity in hepatocytes.

RHOT2 Antibody - N-terminal region (ARP62300_P050) - ARP62300_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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