TBX21

Gene Symbol TBX21
Entrez Gene 30009
Alt Symbol T-PET, T-bet, TBET, TBLYM
Species Human
Gene Type protein-coding
Description T-box 21
Other Description T-box expressed in T cells|T-box protein 21|T-box transcription factor TBX21|T-cell-specific T-box transcription factor T-bet|transcription factor TBLYM
Swissprots Q9UL17
Accessions CAY39299 CAY39301 EAW94804 Q9UL17 AF093098 AAF00055 AF241243 AAF61243 AK223634 BAD97354 BC039739 AAH39739 XM_011524698 XP_011523000 XM_011524699 XP_011523001 NM_013351 NP_037483
Function Transcription factor that controls the expression of the TH1 cytokine, interferon-gamma. Initiates TH1 lineage development from naive TH precursor cells both by activating TH1 genetic programs and by repressing the opposing TH2 programs.
Subcellular Location Nucleus.
Tissue Specificity T-cell specific.
Top Pathways Inflammatory bowel disease (IBD)

T-Bet/TBX21 (D6N8B) XP ® Rabbit mAb (Alexa Fluor ® 647 Conjugate) - 14307 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D6N8B
Confocal immunofluorescent analysis of NK-92 (left, positive) and K-562 (right, negative) cells using T-bet/TBX21 (D6N8B) XP ® Rabbit mAb (Alexa Fluor ® 647 Conjugate) (blue pseudocolor) and β-Actin (8H10D10) Mouse mAb #3700 (red).

T-Bet/TBX21 (D6N8B) XP ® Rabbit mAb (Alexa Fluor ® 488 Conjugate) - 14298 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D6N8B
Confocal immunofluorescent analysis of NK-92 (positive, left) and K-562 (negative, right) cells using T-bet/TBX21 XP ® (D6N8B) Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red).

T-bet/TBX21 (D6N8B) XP ® Rabbit mAb - 13232 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D6N8B
Western blot analysis of extracts from NK-92 and K-562 cells using T-bet/TBX21 (D6N8B) XP ® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

T-bet/TBX21 (V365) Antibody - 5214 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western analysis of extracts from TALL-104 cells using T-bet/TBX21 (V365) Antibody.

T-bet (D-5) - sc-137114 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
T-bet (D-5): sc-137114. Western blot analysis of T-bet expression in non-transfected: sc-117752 (A) and human T-bet transfected: sc-115503 (B) 293T whole cell lysates and BJAB (C) and Jurkat (D) nuclear extracts.

T-bet (N-19) - sc-11330 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Western blot analysis of T-bet expression in BJAB nuclear extract immunoprecipitated with T-bet (4B10): sc-21749 and detected with T-bet (N-19): sc-11330.

T-bet (C-15) - sc-11333 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
T-bet (C-15): sc-11333. Western blot analysis of T-bet expression in non-transfected: sc-117752 (A) and human T-bet transfected: sc-115503 (B) 293T whole cell lysates.

T-bet (39D) - sc-21763 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
T-bet (39D): sc-21763. Western blot analysis of T-bet expression in BJAB nuclear extract.

T-bet (H-210) - sc-21003 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
T-bet (H-210): sc-21003. Immunoperoxidase staining of formalin fixed, paraffin-embedded human tonsil tissue showing nuclear staining of non-follicle cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

T-bet (4B10) - sc-21749 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
T-bet (4B10) PE: sc-21749 PE. Intracellular FCM analysis of fixed and permeabilized human peripheral blood leukocytes. Black line histogram represents the isotype control, normal mouse IgG<sub>1</sub>: sc-2866.

Anti-T-bet / Tbx21 antibody - ab53174 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
T-bet / Tbx21 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab53174.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 53kDa; T-bet / Tbx21

Anti-T-bet / Tbx21 antibody - ab48787 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-T-bet / Tbx21 antibody (ab48787) at 2.5 µg/ml + Fetal thymus lysate at 10 µg with 5% skimmed milk in PBSSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-T-bet / Tbx21 antibody [39D, 3-9D] - ab91103 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 39D, 3-9D
All lanes : Anti-T-bet / Tbx21 antibody [39D, 3-9D] (ab91103) at 2 µg/mlLane 1 : Lysates from control cellsLane 2 : Lysates from PMA and Ionomycin-re-activated cells SecondaryHRP anti-mouse IgG

Anti-T-bet / Tbx21 antibody [4B10] - ab91109 from Abcam

Prices $398.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 4B10
All lanes : Anti-T-bet / Tbx21 antibody [4B10] (ab91109) at 2 µg/mlLane 1 : Lysates of CD4+ T cells (control)Lane 2 : Lysates of PMA and Ionomycin reactivated CD4+ T cellsSecondaryHRP anti-mouse IgG

Anti-T-bet / Tbx21 antibody [EPR9301] - ab150440 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR9301
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human T cell lymphoma tissue labelling T-bet / Tbx21 with ab150440 at 1/250 dilution.

Anti-T-bet / Tbx21 antibody [EPR9302] - ab154200 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR9302
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human Hodgkin's Lymphoma labelling T-bet/Tbx21 with ab154200 at 1/250 dilution

Anti-T-bet / Tbx21 antibody - C-terminal - ab181400 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-T-bet / Tbx21 antibody - C-terminal (ab181400) at 1 µg/ml + 293 cell lysate at 15 µg

Anti-TBX21 / T-bet Antibody (aa8-25) - LS-C3205 from LifeSpan Bioscience

Prices $265.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-TBX21 / T-bet Antibody (aa519-535) - LS-C3206 from LifeSpan Bioscience

Prices $265.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-TBX21 / T-bet Antibody (aa455-504) - LS-C31240 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
SP2/0 Cell Lysate

Anti-TBX21 / T-bet Antibody (aa36-85) - LS-C31246 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Fetal Thymus Lysate

Anti-TBX21 / T-bet Antibody (CF) - LS-C126357 from LifeSpan Bioscience

Prices $445.00
Sizes 100 tst
Host Mouse
Clonality Monoclonal

Anti-TBX21 / T-bet Antibody (clone 39D, 3-9D) - LS-C106602 from LifeSpan Bioscience

Prices $225.00, $355.00
Sizes 25 µg, 100 µg
Host Mouse
Clonality Monoclonal
CD4+ T cells were sorted from mouse spleen, activated with anti-mouse CD3 and anti-mouse CD28, followed by culture in Th1-polarizing conditions, and re-activation with PMA and Ionomycin. Lysates from control (left) or PMA and Ionomycin-re-activated (right) cells were probed with purified antibody at 2 ug/ml, and revealed with HRP anti-mouse IgG.

Anti-TBX21 / T-bet Antibody - LS-C41297 from LifeSpan Bioscience

Prices $245.00, $345.00
Sizes 50 µl, 200 µl
Host Rabbit
Clonality Polyclonal
Hela nuclear extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-T-bet antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.

Anti-TBX21 / T-bet Antibody (clone 12E4.1) - LS-C190152 from LifeSpan Bioscience

Prices $445.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 12E4.1
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