KMT2E

Gene Symbol KMT2E
Entrez Gene 55904
Alt Symbol HDCMC04P, MLL5, NKp44L
Species Human
Gene Type protein-coding
Description lysine (K)-specific methyltransferase 2E
Other Description histone-lysine N-methyltransferase 2E|histone-lysine N-methyltransferase MLL5|myeloid/lymphoid or mixed-lineage leukemia 5 (trithorax homolog, Drosophila)|myeloid/lymphoid or mixed-lineage leukemia protein 5
Swissprots Q9NWE7 Q6PKG4 B6ZDE4 Q86WG0 Q86TI3 M4K8J3 B6ZDM3 Q8IZD2 Q6P5Y2 Q8IWR5 Q8IV78 Q8NFF8 Q86W12 Q86WL2 Q6T316
Accessions AAD04721 EAW83355 EAW83356 EAW83357 EAW83358 Q8IZD2 AF067804 AAF75564 AF519459 AAM74947 AK000940 AK024088 BAB14821 AY113691 AY147037 AAN17675 AY157990 AAN76325 AY195568 AAO47009 AY195569 AAO47010 AY211525 AAO86416 AY222296 AAO64395 AY234382 AAO89072 AY438698 AAR13893 AY438699 AAR13894 BC001296 AAH01296 BC009061 BC016382 BC024658 BC040004 AAH40004 BC040848 BC053906 AAH53906 BC062583 AAH62583 BC142987 AAI42988 JQ809698 AGE34449 XM_005250493 XP_005250550 XM_006716049 XP_006716112 XM_011516400 XP_011514702 XR_927489 NM_018682 NP_061152 NM_182931 NP_891847 NM_032187
Function Histone methyltransferase that specifically mono- and dimethylates 'Lys-4' of histone H3 (H3K4me1 and H3K4me2). H3 'Lys- 4' methylation represents a specific tag for epigenetic transcriptional activation. Key regulator of hematopoiesis involved in terminal myeloid differentiation and in the regulation of hematopoietic stem cell (HSCs) self-renewal by a mechanism that involves DNA methylation. Plays an essential role in retinoic- acid-induced granulopoiesis by acting as a coactivator of RAR- alpha (RARA) in target gene promoters. Also acts as an important cell cycle regulator, participating in cell cycle regulatory network machinery at multiple cell cycle stages. Required to suppress inappropriate expression of S-phase-promoting genes and maintain expression of determination genes in quiescent cells. Overexpression inhibits cell cycle progression, while knockdown induces cell cycle arrest at both the G1 and G2/M phases.
Subcellular Location Nucleus speckle {ECO:0000269|PubMed:14718661}. Note=Absent from the nucleolus. {ECO:0000269|PubMed:14718661}.
Tissue Specificity Widely expressed in both adult and fetal tissues. Highest levels of expression observed in fetal thymus and kidney and in adult hematopoietic tissues, jejunum and cerebellum. Isoform NKp44L is not detected on circulating cells from healthy individuals, but it is expressed on a large panel of the tumor and transformed cells. {ECO:0000269|PubMed:12101424, ECO:0000269|PubMed:23958951}.
Top Pathways Lysine degradation

MLL5 (C-10) - sc-377182 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MLL5 (C-10): sc-377182. Western blot analysis of MLL5 expression in HL-60 whole cell lysate.

MLL5 (H-110) - sc-292360 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

MLL5 (L-14) - sc-68635 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MLL5 (L-14): sc-68635. Immunoperoxidase staining of formalin fixed, paraffin-embedded human bone marrow tissue showing cytoplasmic and nuclear staining of hematopoietic cells.

MLL5 (N-20) - sc-68636 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MLL5 (N-20): sc-68636. Western blot analysis of MLL5 expression in mouse cerebellum (A) and mouse brain (B) tissue extracts.

Anti-MLL5 antibody - ab75339 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab75339 staining MLL5 in HeLa cells by Immunocytochemistry/ Immunoflourescence. Cells were fixed in Methanol and permeabilized in PHEM/0.5% Triton X-100 prior to blocking in 2.5% BSA for 1 hour at 21°C. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 21°C. The secondary antibody was FITC-conjugated Donkey anti-Rabbit polyclonal, diluted 1/150. DNA (DAPI) stain in blue, anti-Aurora A antibody in red, anti-MLL5 in green. Left cell shows centrosomal staining in interphase (arrow head). Right cell shows centrosomal staining in mitotic cell. Centrosomal localisation of MLL5 was shown in Liu et al (2010) PMID:20439461See Abreview

Anti-MLL5 antibody - ab192582 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-MLL5 antibody - ab168138 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-MLL5 antibody (ab168138) at 1 µg/mlLane 1 : MLL5 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Anti-MLL5 antibody - ab123124 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-MLL5 antibody (ab123124) at 1/100 dilution + HL60 cell line lysates at 35 µg

Anti-KMT2E / MLL5 Antibody (N-Terminus) - LS-C144904 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-KMT2E / MLL5 Antibody (aa92-121, AP) - LS-C237689 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-KMT2E / MLL5 Antibody (aa92-121, APC) - LS-C237687 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-KMT2E / MLL5 Antibody (aa92-121, Azide-free, HRP) - LS-C248828 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-KMT2E / MLL5 Antibody (aa92-121, PE) - LS-C237694 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-KMT2E / MLL5 Antibody (aa92-121, Biotin) - LS-C237688 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-KMT2E / MLL5 Antibody (aa92-121, FITC) - LS-C237690 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-KMT2E / MLL5 Antibody - LS-C310992 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-KMT2E / MLL5 Antibody - LS-C309016 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-KMT2E / MLL5 Antibody (aa92-121) - LS-C329836 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-KMT2E / MLL5 Antibody (aa93-120) - LS-C164217 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
MLL5 Antibody western blot of HL-60 cell line lysates (35 ug/lane). The MLL5 antibody detected the MLL5 protein (arrow).

Anti-KMT2E / MLL5 Antibody - LS-C341940 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-KMT2E / MLL5 Antibody - LS-C334521 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines, using MLL5 antibody.

Anti-KMT2E / MLL5 Antibody IHC-plus™ - LS-B867 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-MLL5 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B867 concentration 10 ug/ml.

MLL5 Antibody - NBP2-33775 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry-Paraffin: MLL5 Antibody [NBP2-33775] - Staining of human bone marrow shows strong positivity in a subset of hematopoietic cells.

MLL5 Antibody - NBP1-18697 from Novus Biologicals

Prices $329.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

MLL5 Antibody - NBP1-02511 from Novus Biologicals

Prices $329.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry-Paraffin: MLL5 Antibody [NBP1-02511] - Staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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