PBK

Gene Symbol PBK
Entrez Gene 55872
Alt Symbol CT84, HEL164, Nori-3, SPK, TOPK
Species Human
Gene Type protein-coding
Description PDZ binding kinase
Other Description MAPKK-like protein kinase|T-LAK cell-originated protein kinase|cancer/testis antigen 84|epididymis luminal protein 164|lymphokine-activated killer T-cell-originated protein kinase|serine/threonine protein kinase|spermatogenesis-related protein kinase
Swissprots Q96KB5 B4DX68 Q9NPD9 Q9NZK6 Q9NYL7 D3DST2
Accessions CAD43677 EAW63536 EAW63537 Q96KB5 AB027249 BAA99576 AB027250 BAA99577 AF189722 AAF69107 AF237709 AAF71521 AK027291 BAB55019 AK301836 BAG63280 BC015191 AAH15191 BI461026 DB028500 DQ893142 ABM84068 DQ896427 ABM87426 EU794685 ACJ13739 XM_006716368 XP_006716431 NM_001278945 NP_001265874 NM_018492 NP_060962
Function Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage. {ECO:0000269|PubMed:10781613, ECO:0000269|PubMed:17482142}.
Tissue Specificity Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules. {ECO:0000269|PubMed:10781613, ECO:0000269|PubMed:11378444}.

Phospho-PBK/TOPK (Thr9) Antibody - 4941 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HT29 cells, untreated or nocodazole-treated (50ng/ml), using Phospho-PBK/TOPK (Thr9) Antibody (upper) or PBK/TOPK Antibody #4942 (lower).

PBK/TOPK Antibody - 4942 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from NIH/3T3 and PC12 cells, using PBK/TOPK Antibody.

PBK (A-3) - sc-393313 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PBK (A-3): sc-393313. Western blot analysis of PBK expression in Jurkat (A) and HeLa (B) whole cell lysates.

PBK (A-6) - sc-390817 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PBK (A-6): sc-390817. Western blot analysis of PBK expression in SW480 (A), Jurkat (B), PC-12 (C) and HeLa (D) whole cell lysates.

PBK (B-10) - sc-390399 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PBK (B-10): sc-390399. Western blot analysis of PBK expression in Jurkat whole cell lysate (A) and mouse testis tissue extract (B).

PBK (C-3) - sc-390812 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PBK (C-3): sc-390812. Western blot analysis of PBK expression in HeLa (A) and Jurkat (B) whole cell lysates.

PBK (F-7) - sc-390790 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PBK (F-7): sc-390790. Western blot analysis of PBK expression in HeLa (A) and Jurkat (B) whole cell lysates and mouse testis tissue extract (C).

PBK (H-9) - sc-390400 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PBK (H-9): sc-390400. Western blot analysis of PBK expression in Jurkat whole cell lysate (A) and mouse testis (B) and human testis (C) tissue extracts. Note the lack of reactivity with mouse PBK in lane B.

PBK (11) - sc-293028 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PBK (11): sc-293028. Western blot analysis of PBK expression in A-431 (A), Jurkat (B) and PC-12 (C) whole cell lysates and mouse testis tissue extract (D).

PBK (31) - sc-136026 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
PBK (31): sc-136026. Western blot analysis of PBK expression in Jurkat whole cell lysate.

PBK (H-152) - sc-366752 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

PBK (C-17)-R - sc-15243-R from Santa Cruz Biotechnology

Prices $61.00
Sizes 100 µg/0.5 ml
Host Rabbit
Clonality Polyclonal
PBK (C-17): sc-15243. Western blot analysis of PBK expression in A-431 whole cell lysate.

PBK (H-139) - sc-292890 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PBK (H-139): sc-292890. Western blot analysis of PBK expression in Jurkat whole cell lysate (A) and mouse testis tissue extract (B).

Anti-PBK (phospho T9) antibody - ab63363 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PBK (phospho T9) antibody (ab63363) at 1/500 dilutionLane 1 : K562 cells, treated with UV (30mins); without the phosphopeptideLane 2 : K562 cells, treated with UV (30mins); with the phosphopeptide (5 µg)Lysates/proteins at 5 µg per lane.

Anti-PBK (phospho T9) antibody [EPR2475(N)] - ab184953 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2475(N)
All lanes : Anti-PBK (phospho T9) antibody [EPR2475(N)] (ab184953) at 1/5000 dilutionLane 1 : Hela lysate treated with NocodazoleLane 2 : Hela lysate untreatedLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Anti-PBK antibody - ab88326 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Anti-PBK antibody (ab88326) at 1 µg/ml + HeLa NE cell lysate at 25 µg

Anti-PBK antibody - ab59327 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Ab59327 at 1/50-1/100 dilution staining human brain tissue without (left) and with (right) immunizing peptide; paraffin embedded.

Anti-PBK antibody - ab75494 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-PBK antibody (ab75494) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-PBK antibody - ab57663 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-PBK antibody [EP2519Y] - ab75983 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2519Y
Anti-PBK antibody [EP2519Y] (ab75983) at 1/100000 dilution + HeLa cell lysate at 10 µgSecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-PBK antibody [EP2520Y] - ab75987 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2520Y
ab75987 stained JEG-3 cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75987 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Anti-PBK antibody - C-terminal - ab190075 from Abcam

Prices $407.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon tissue labeling PBK with ab190075 at 10µg/ml.

Anti-PBK antibody - C-terminal - ab191146 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PBK antibody - C-terminal (ab191146) at 0.5 µg/mlLane 1 : Rat testis tissue lysateLane 2 : HeLa cell lysateLane 3 : MCF7 cell lysateLane 4 : SKOV cell lysateLane 5 : HepG2 cell lysateLane 6 : COLO320 cell lysateLysates/proteins at 40 µg per lane.

Anti-PBK antibody - N-terminal - ab174485 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-PBK antibody - N-terminal (ab174485) at 1/100 dilution + MDA-MB453 cell line lysate at 35 µg

Anti-PBK / TOPK Antibody (N-Terminus, clone EP2519Y) - LS-C50199 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2519Y
Western blot analysis on HeLa cell lysate using anti-PBK antibody, dilution 1:100000.
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