SKP2

Gene Symbol	SKP2
Entrez Gene	6502
Alt Symbol	FBL1, FBXL1, FLB1, p45
Species	Human
Gene Type	protein-coding
Description	S-phase kinase-associated protein 2, E3 ubiquitin protein ligase
Other Description	CDK2/cyclin A-associated protein p45\|F-box/LRR-repeat protein 1\|S-phase kinase-associated protein 2\|S-phase kinase-associated protein 2 (p45)\|p45skp2
Swissprots	Q8TDZ1 Q13309 A8K5E0 Q8TDZ0 Q9BV69 B4DJT4
Accessions	AAY87142 EAW55934 EAW55935 EAW55936 EAW55937 EAW55938 Q13309 AB050979 BAB87200 AB050980 BAB87201 AB050981 BAB87202 AK026224 AK291255 BAF83944 AK295152 BAG58168 AK296223 BAG58946 AY029177 AAK31593 BC001441 AAH01441 BC007441 AAH07441 CA415778 DA223617 U33761 AAC50242 XM_011514081 XP_011512383 XM_011514082 XP_011512384 XM_011514083 XP_011512385 NM_001243120 NP_001230049 NM_005983 NP_005974 NM_032637 NP_116026
Function	Substrate recognition component of a SCF (SKP1-CUL1-F- box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, KMT2A/MLL1, CDK9, RAG2, FOXO1, UBP43, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2. Promotes ubiquitination and destruction of CDH1 in a CK1-Dependent Manner, thereby regulating cell migration. {ECO:0000269\|PubMed:11931757, ECO:0000269\|PubMed:12435635, ECO:0000269\|PubMed:12769844, ECO:0000269\|PubMed:12840033, ECO:0000269\|PubMed:15342634, ECO:0000269\|PubMed:15668399, ECO:0000269\|PubMed:15949444, ECO:0000269\|PubMed:16103164, ECO:0000269\|Pu
Subcellular Location	Cytoplasm {ECO:0000269\|PubMed:22770219}. Nucleus {ECO:0000269\|PubMed:22770219}.
Top Pathways	Cell cycle, Ubiquitin mediated proteolysis, Small cell lung cancer, Herpes simplex infection, Viral carcinogenesis

Phospho-Skp2 (Ser64) Antibody - 14865 from Cell Signaling Technology

Prices	$287.00
Sizes	100 µl (10 western blots)
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of extracts from HeLa cells expressing either non-targeting shRNA (shNT) or Skp2 shRNA (shSkp2) using Phospho-Skp2 (Ser64) Antibody (upper), Skp2 (D3G5) XP ® Rabbit mAb #2652 (middle), and GAPDH (D16H11) XP ® Rabbit mAb #5174 (lower).

Skp2 (D3G5) XP ® Rabbit mAb - 2652 from Cell Signaling Technology

Prices	$109.00, $277.00
Sizes	20 µl (2 western blots), 100 µl (10 western blots)
Host	Rabbit
Clonality	Monoclonal
Clone	D3G5

Western blot analysis of extracts from various cell lines using Skp2 (D3G5) XP ® Rabbit mAb.

Skp2 Antibody - 4358 from Cell Signaling Technology

Prices	$99.00, $246.00
Sizes	20 µl (2 western blots), 100 µl (10 western blots)
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of extracts from HeLa, K562 and COS cells using Skp2 Antibody.

Skp2 (L70) Antibody - 4313 from Cell Signaling Technology

Prices	$246.00
Sizes	100 µl (10 western blots)
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of extracts from various cell lines using Skp2 (L70) Antibody.

Skp2 p45 (A-2) - sc-74477 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Mouse
Clonality	Monoclonal

Skp2 p45 (A-2): sc-74477. Western blot analysis of Skp2 p45 expression in SK-N-MC whole cell lysate (A) and A-673 (B) and K-562 (C) nuclear extracts.

Skp2 p45 (H-435) - sc-7164 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

Skp2 p45 (H-435): sc-7164. Western blot analysis of Skp2 p45 expression in SK-N-MC whole cell lysate (A) and A-673 (B) and K-562 (C) nuclear extracts.

Skp2 p45 (N-19) - sc-1567 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Skp2 p45 (N-19): sc-1567. Western blot analysis of Skp2 p45 expression in A-673 (A), K-562 (B), Jurkat (C), MCF7 (D) and KNRK (E) nuclear extracts.

Anti-SKP2 antibody - ab68455 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

ab68455 at 1µg/ml staining SKP2 in Human mammary cancer tissue sections by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). The tissue underwent enzymatic antigen retrieval in pepsin and trypsin. A Biotin-conjugated Goat anti-rabbit IgG was used as secondary at 1/200 dilution.

Anti-SKP2 antibody - ab19877 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-SKP2 antibody (ab19877) at 1 µg/mlLane 1 : Human heart tissue lysate Lane 2 : Human brain tissue lysateLane 3 : Human kidney normal tissue lysate Lane 4 : Human testis tissue lysate Lysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilutionPerformed under reducing conditions.

Anti-SKP2 antibody - ab135691 from Abcam

Prices	$370.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

Anti-SKP2 antibody (ab135691) at 1/50 dilution + HeLa cell line lysates at 35 µg

Anti-SKP2 antibody - ab87809 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Lane 1: ab87809 (1µg/ml) immunoprecipitating SKP2 in HeLa Whole Cell Lysate (1mg for IP, 20% loaded). Lane 2: positive control IP (done using an antibody that recognises downstream epitope). Lane 3: Control IgG

Anti-SKP2 antibody - ab53722 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-SKP2 antibody (ab53722) at 1/500 dilutionLane 1 : Extracts from 293 cellsLane 2 : Extracts from 293 cells with immunizing peptide

Anti-SKP2 antibody [EPR3305(2)] - ab183039 from Abcam

Prices	$357.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR3305(2)

Flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling SKP2 with ab183039 at 1/70 followed by Goat anti rabbit IgG (FITC) at 1/150. Rabbit monoclonal IgG was used as Isotype control.

Anti-SKP2 antibody [EPR3306(2)] - ab124799 from Abcam

Prices	$313.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR3306(2)

All lanes : Anti-SKP2 antibody [EPR3306(2)] (ab124799) at 1/1000 dilutionLane 1 : 293T cell lysates Lane 2 : HeLa cell lysates Lane 3 : SW480 cell lysates Lane 4 : LnCaP cell lysates Lysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-SKP2 Antibody (aa2-20) - LS-C122445 from LifeSpan Bioscience

Prices	$445.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-SKP2 Antibody (N-Terminus) - LS-C122446 from LifeSpan Bioscience

Prices	$405.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-SKP2 Antibody (clone 6G9D10) - LS-C109113 from LifeSpan Bioscience

Prices	$345.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	6G9D10

Western blot using SKP2 mouse monoclonal antibody against truncated Trx-SKP2 recombinant protein (1) and GST-SKP2 (aa1-130) recombinant protein (2).

Anti-SKP2 Antibody (N-Terminus, Magnetic beads) - LS-C171854 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-SKP2 Antibody (N-Terminus, Sepharose) - LS-C171855 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-SKP2 Antibody (clone 1G12E9) - LS-C202805 from LifeSpan Bioscience

Prices	$670.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	1G12E9

Anti-SKP2 Antibody (aa1-50) - LS-C289171 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-SKP2 Antibody (aa50-100) - LS-C289174 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-SKP2 Antibody (aa53-320) - LS-C311718 from LifeSpan Bioscience

Prices	$250.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-SKP2 Antibody (Biotin) - LS-C343031 from LifeSpan Bioscience

Prices	$305.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Anti-SKP2 Antibody - LS-C343541 from LifeSpan Bioscience

Prices	$275.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

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