ALKBH5

Gene Symbol ALKBH5
Entrez Gene 54890
Alt Symbol ABH5, OFOXD, OFOXD1
Species Human
Gene Type protein-coding
Description AlkB family member 5, RNA demethylase
Other Description RNA demethylase ALKBH5|alkB, alkylation repair homolog 5|alkylated DNA repair protein alkB homolog 5|alpha-ketoglutarate-dependent dioxygenase alkB homolog 5|oxoglutarate and iron-dependent oxygenase domain containing|probable alpha-ketoglutarate-dependent dioxygenase ABH5
Swissprots D3DXC6 Q6P6C2 Q9NXD6 B4DVJ4
Accessions EAW55661 EAW55662 Q6P6C2 AK000129 AK000315 BAA91078 AK025817 AK301107 BAG62706 AK311161 AK311511 AL137263 BC062339 AAH62339 BI823385 BU740432 NM_017758 NP_060228
Function Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes (PubMed:23177736, PubMed:24778178, PubMed:24616105, PubMed:24489119). Can also demethylate N(6)- methyladenosine in single-stranded DNA (in vitro) (PubMed:24616105). Requires molecular oxygen, alpha-ketoglutarate and iron (PubMed:21264265, PubMed:23177736, PubMed:24778178, PubMed:24616105, PubMed:24489119). Demethylation of m6A mRNA affects mRNA processing and export (PubMed:23177736). Required for spermatogenesis (By similarity). {ECO:0000250|UniProtKB:Q3TSG4, ECO:0000269|PubMed:21264265, ECO:0000269|PubMed:23177736, ECO:0000269|PubMed:24489119, ECO:0000269|PubMed:24616105, ECO:0000269|PubMed:24778178}.
Subcellular Location Nucleus speckle {ECO:0000269|PubMed:21264265, ECO:0000269|PubMed:23177736}.
Tissue Specificity Widely expressed, with highest expression in lung, followed by testis, pancreas, spleen and ovary. {ECO:0000269|PubMed:17979886}.

Anti-ALKBH5 antibody - ab69325 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-ALKBH5 antibody (ab69325) at 1/500 dilutionLane 1 : ALKBH5 transfected 293T cell lysateLane 2 : Non transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Anti-ALKBH5 Antibody (aa302-330) - LS-C161042 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
ALKBH5 Antibody western blot of 293 cell line lysates (35 ug/lane). The ALKBH5 Antibody detected the ALKBH5 protein (arrow).

Anti-ALKBH5 Antibody (aa302-330, PE) - LS-C251212 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ALKBH5 Antibody (aa302-330, FITC) - LS-C251211 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ALKBH5 Antibody (aa302-330, Azide-free, HRP) - LS-C251213 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ALKBH5 Antibody (aa302-330, APC) - LS-C252850 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ALKBH5 Antibody (aa302-330, AP) - LS-C252849 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ALKBH5 Antibody - LS-C306211 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-ALKBH5 Antibody (aa302-330, Biotin) - LS-C252851 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ALKBH5 Antibody (aa301-330) - LS-C319435 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

ALKBH5 Antibody - NBP1-82188 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: ALKBH5 Antibody [NBP1-82188] - Analysis in human cell line U-87 MG.

ALKBH5 Antibody - H00054890-B01 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: ALKBH5 Antibody [H00054890-B01] - analysis of OFOXD expression in transfected 293T cell line by OFOXD MaxPab polyclonal antibody (B01). Lane 1: OFOXD transfected lysate (51.40 KDa). Lane 2: Non-transfected lysate.

ALKBH5 Antibody - H00054890-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: ALKBH5 Antibody [H00054890-B01P] - Analysis of ALKBH5 expression in transfected 293T cell line by ALKBH5 polyclonal antibody. Lane 1: OFOXD transfected lysate(50.38 KDa). Lane 2: Non-transfected lysate.

Anti-ALKBH5 antibody produced in mouse - SAB1407587 from Sigma-Aldrich

Prices $347.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-ALKBH5: <B>Cat. No. SAB1407587</B>: Antibody concentration: 0.1-5 μg/mL. Specific band of ~50.4 kDa using ALKBH5 transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.

Anti-ALKBH5 antibody produced in rabbit - HPA007196 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-ALKBH5: <B>Cat. No. HPA007196</B>: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human duodenum shows strong cytoplasmic and nuclear positivity in glandular cells.

ALKBH5-Antibody-Center - AP18410c from Abgent, a WuXi AppTec company

Prices $99.00, $295.00
Sizes 80 µl, 400 µl
Host Rabbit
Clonality Polyclonal
Anti-ALKBH5 Antibody (Center) at 1:2000 dilution + 293 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

ALKBH5 Antibody - middle region (OAAB13944) - OAAB13944 from Aviva Systems Biology

Prices $289.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
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