Gene Symbol | ALKBH5 |
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Entrez Gene | 54890 |
Alt Symbol | ABH5, OFOXD, OFOXD1 |
Species | Human |
Gene Type | protein-coding |
Description | AlkB family member 5, RNA demethylase |
Other Description | RNA demethylase ALKBH5|alkB, alkylation repair homolog 5|alkylated DNA repair protein alkB homolog 5|alpha-ketoglutarate-dependent dioxygenase alkB homolog 5|oxoglutarate and iron-dependent oxygenase domain containing|probable alpha-ketoglutarate-dependent dioxygenase ABH5 |
Swissprots | D3DXC6 Q6P6C2 Q9NXD6 B4DVJ4 |
Accessions | EAW55661 EAW55662 Q6P6C2 AK000129 AK000315 BAA91078 AK025817 AK301107 BAG62706 AK311161 AK311511 AL137263 BC062339 AAH62339 BI823385 BU740432 NM_017758 NP_060228 |
Function | Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes (PubMed:23177736, PubMed:24778178, PubMed:24616105, PubMed:24489119). Can also demethylate N(6)- methyladenosine in single-stranded DNA (in vitro) (PubMed:24616105). Requires molecular oxygen, alpha-ketoglutarate and iron (PubMed:21264265, PubMed:23177736, PubMed:24778178, PubMed:24616105, PubMed:24489119). Demethylation of m6A mRNA affects mRNA processing and export (PubMed:23177736). Required for spermatogenesis (By similarity). {ECO:0000250|UniProtKB:Q3TSG4, ECO:0000269|PubMed:21264265, ECO:0000269|PubMed:23177736, ECO:0000269|PubMed:24489119, ECO:0000269|PubMed:24616105, ECO:0000269|PubMed:24778178}. |
Subcellular Location | Nucleus speckle {ECO:0000269|PubMed:21264265, ECO:0000269|PubMed:23177736}. |
Tissue Specificity | Widely expressed, with highest expression in lung, followed by testis, pancreas, spleen and ovary. {ECO:0000269|PubMed:17979886}. |
ALKBH5 CRISPR/Cas9 KO Plasmid (h) - sc-412371 from Santa Cruz Biotechnology
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ALKBH5 HDR Plasmid (h) - sc-412371-HDR from Santa Cruz Biotechnology
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ALKBH5 Double Nickase Plasmid (h) - sc-412371-NIC from Santa Cruz Biotechnology
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ALKBH5 Double Nickase Plasmid (h2) - sc-412371-NIC-2 from Santa Cruz Biotechnology
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ALKBH5 CRISPR Activation Plasmid (h) - sc-412371-ACT from Santa Cruz Biotechnology
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ALKBH5 CRISPR Activation Plasmid (h2) - sc-412371-ACT-2 from Santa Cruz Biotechnology
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ALKBH5 Lentiviral Activation Particles (h) - sc-412371-LAC from Santa Cruz Biotechnology
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ALKBH5 Lentiviral Activation Particles (h2) - sc-412371-LAC-2 from Santa Cruz Biotechnology
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ALKBH5 siRNA (h) - sc-93856 from Santa Cruz Biotechnology
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ALKBH5 shRNA Plasmid (h) - sc-93856-SH from Santa Cruz Biotechnology
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ALKBH5 shRNA (h) Lentiviral Particles - sc-93856-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU021751 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN3070 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND3070 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR10105 from Sigma-Aldrich
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ALKBH5 siRNA (Human) - CRJ0300 from Cohesion Biosciences
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ALKBH5 - MBS8217361 from MyBioSource
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shRNA set against Human ALKBH5 (NM_017758.3) - SHH235258 from Creative biogene
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shRNA set against Human ALKBH5(NM_017758.3) - SHG054433 from Creative biogene
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Human ALKBH5 siRNA - orb270801 from Biorbyt
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ALKBH5 shRNA Plasmid (Human) - CHJ0300 from Cohesion Biosciences
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ALKBH5 miRNA 3'UTR clone - MiUTR1H-00307 from Creative biogene
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