MAP4K2
| Gene Symbol | MAP4K2 |
|---|---|
| Entrez Gene | 5871 |
| Alt Symbol | BL44, GCK, RAB8IP |
| Species | Human |
| Gene Type | protein-coding |
| Description | mitogen-activated protein kinase kinase kinase kinase 2 |
| Other Description | B lymphocyte serine/threonine protein kinase|MAPK/ERK kinase kinase kinase 2|MEK kinase kinase 2|MEKKK 2|Rab8 interacting protein|germinal centre kinase (GC kinase) |
| Swissprots | Q86VU3 Q12851 |
| Accessions | EAW74299 EAW74300 EAW74301 EAW74302 Q12851 AK298383 BAG60619 AK304495 BAG65303 AW574847 BC039839 BC047865 AAH47865 BM458161 BU844790 HY168726 U07349 AAA20968 XM_011545202 XP_011543504 XM_011545203 XP_011543505 XM_011545204 XP_011543506 NM_001307990 NP_001294919 NM_004579 NP_004570 |
| Function | Serine/threonine-protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Acts as a MAPK kinase kinase kinase (MAP4K) and is an upstream activator of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway and to a lesser extend of the p38 MAPKs signaling pathway. Required for the efficient activation of JNKs by TRAF6-dependent stimuli, including pathogen-associated molecular patterns (PAMPs) such as polyinosine-polycytidine (poly(IC)), lipopolysaccharides (LPS), lipid A, peptidoglycan (PGN), or bacterial flagellin. To a lesser degree, IL-1 and engagement of CD40 also stimulate MAP4K2-mediated JNKs activation. The requirement for MAP4K2/GCK is most pronounced for LPS signaling, and extends to LPS stimulation of c-Jun phosphorylation and induction of IL-8. Enhances MAP3K1 oligomerization, which may relieve N-terminal mediated MAP3K1 autoinhibition and lead to activation following autophosphorylation. Mediates also |
| Subcellular Location | Cytoplasm {ECO:0000250}. Basolateral cell membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}. Golgi apparatus membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}. |
| Tissue Specificity | Highly expressed in germinal center but not mantle zone B-cells. Also expressed in lung, brain and placenta and at lower levels in other tissues examined. {ECO:0000269|PubMed:7515885}. |
| Top Pathways | MAPK signaling pathway |
GC kinase CRISPR/Cas9 KO Plasmid (h) - sc-405766 from Santa Cruz Biotechnology
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GC kinase HDR Plasmid (h) - sc-405766-HDR from Santa Cruz Biotechnology
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GC kinase Double Nickase Plasmid (h) - sc-405766-NIC from Santa Cruz Biotechnology
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GC kinase Double Nickase Plasmid (h2) - sc-405766-NIC-2 from Santa Cruz Biotechnology
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GC kinase CRISPR Activation Plasmid (h) - sc-405766-ACT from Santa Cruz Biotechnology
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GC kinase CRISPR Activation Plasmid (h2) - sc-405766-ACT-2 from Santa Cruz Biotechnology
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GC kinase Lentiviral Activation Particles (h) - sc-405766-LAC from Santa Cruz Biotechnology
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GC kinase Lentiviral Activation Particles (h2) - sc-405766-LAC-2 from Santa Cruz Biotechnology
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GC kinases siRNA (h) - sc-39239 from Santa Cruz Biotechnology
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GC kinases shRNA Plasmid (h) - sc-39239-SH from Santa Cruz Biotechnology
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GC kinases shRNA (h) Lentiviral Particles - sc-39239-V from Santa Cruz Biotechnology
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MAP4K2 RNAi - H00005871-R02 from Novus Biologicals
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MISSION® esiRNA - EHU007141 from Sigma-Aldrich
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MISSION® siRNA - SIHK1173 from Sigma-Aldrich
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MISSION® siRNA - SIHK1171 from Sigma-Aldrich
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MISSION® siRNA - SIHK1172 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN13000 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND13000 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR03166 from Sigma-Aldrich
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MAP4K2 siRNA (Human) - CRH3961 from Cohesion Biosciences
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shRNA set against Human MAP4K2 (NM_004579.3) - SHH337811 from Creative biogene
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MAP4K2 shRNA Plasmid (Human) - CHH3961 from Cohesion Biosciences
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MAP4K2 miRNA 3'UTR clone - MiUTR3H-05732 from Creative biogene
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