SMAD1

Gene Symbol SMAD1
Entrez Gene 4086
Alt Symbol BSP-1, BSP1, JV4-1, JV41, MADH1, MADR1
Species Human
Gene Type protein-coding
Description SMAD family member 1
Other Description MAD homolog 1|MAD, mothers against decapentaplegic homolog 1|Mad-related protein 1|SMAD, mothers against DPP homolog 1|TGF-beta signaling protein 1|mothers against DPP homolog 1|mothers against decapentaplegic homolog 1|transforming growth factor-beta signaling protein 1|transforming growth factor-beta-signaling protein 1
Swissprots A8KAJ0 Q9UFT8 Q15797 Q16636 D3DNZ9
Accessions CAB55898 CBY84968 EAX05037 EAX05038 EAX05039 EAX05040 Q15797 AK096624 AK293055 BAF85744 BC001878 AAH01878 BE644607 BI772689 BI792941 BQ962660 BT007386 AAP36050 BU163870 CB956339 DQ892510 ABM83436 DQ895724 ABM86650 U54826 AAC50493 U57456 AAC50621 U59423 AAB06852 U59912 AAC50790 XM_005262991 XP_005263048 XM_005262992 XP_005263049 XM_005262993 XP_005263050 XM_006714217 XP_006714280 XM_011531961 XP_011530263 XM_011531962 XP_011530264 XM_011531963 XP_011530265 XM_011531964 XP_011530266 NM_001003688 NP_001003688 NM_005900 NP_005891
Function Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. {ECO:0000269|PubMed:12097147}.
Subcellular Location Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane.
Tissue Specificity Ubiquitous. Highest expression seen in the heart and skeletal muscle.
Top Pathways TGF-beta signaling pathway, Hippo signaling pathway, Signaling pathways regulating pluripotency of stem cells, Transcriptional misregulation in cancer

SMAD1 (pS463/465) PhosphoTracer ELISA Kit - ab119647 from Abcam

Category ELISA Kit
Prices $489.00
Sizes 1 x 96 tests
Sensitivity 5000 cells/well
Range 5000 cells/well - 100000 cells/well
Using the SMAD1 assay kits or Western blot, SMAD1 phosphorylation at Ser463/465 is detected in BMP4-treated C2C12 cells (+), compared with C2C12 cells treated with 50 µM dorsomorphin (-). Upon phosphorylation, SMAD1 levels decrease as SMAD1 becomes ubiquitinated.

SMAD1 (pS463/465) SimpleStep ELISA™ Kit - ab186036 from Abcam

Category ELISA Kit
Prices $484.00
Sizes 1 x 96 tests
Sensitivity < 10 µg/ml
Range 10 µg/ml - 500 µg/ml
Example of a typical SMAD1 (pS463/S465) cell lysate dilution series. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

SMAD1 (pS463/S465) + Total SMAD1 SimpleStep ELISA™ Kit - ab186035 from Abcam

Category ELISA Kit
Prices $484.00
Sizes 1 x 96 tests
Sensitivity < 20 µg/ml
Range 10 µg/ml - 1000 µg/ml
Example of a typical SMAD1 (pS463/S465) and SMAD1 (Total) cell lysate dilution series. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Using Western blot, SMAD1 phosphorylation at Ser463/465 is detected in BMP4-treated C2C12 cells (+), compared with C2C12 cells treated with 50 µM dorsomorphin (-). Upon phosphorylation, SMAD1 levels decrease as SMAD1 becomes ubiquitinated.

SMAD1 SimpleStep ELISA™Kit - ab186037 from Abcam

Category ELISA Kit
Prices $484.00
Sizes 1 x 96 tests
Sensitivity < 20 ng/ml
Range 20 µg/ml - 1000 µg/ml
Example of a typical SMAD1 cell lysate dilution series. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

SMAD1 Total PhosphoTracer ELISA Kit - ab119648 from Abcam

Category ELISA Kit
Sensitivity 10000 cells/well
Range 10000 cells/well - 100000 cells/well
C2C12 cells were seeded at 5K cells/well in a 96 well tissue culture microplate overnight. The next day cells were serum starved for 60 minutes, then treated with various concentrations of dorsomorphin for 30 mins. Subsequently, the cells stimulated with 50 ng/mL BMP-4 for 30 min. The medium was removed from the wells, and cells were lysed with 120 µl/well of Lysis Mix, with shaking for 10 min. The lysates were transferred to a PhosphoTracer assay plate and assayed for either total or phospho-SMAD1, using the standard protocol. Signal in the wells was determined using a plate reader.

Mouse/Human/Rat SMAD1 ELISA Kit (Cell-Based ELISA) - LS-F2311 from LifeSpan Bioscience

Category ELISA Kit
Prices $450.00
Sizes 1 plate

Mouse/Human/Rat Phospho-SMAD1 (Ser187) ELISA Kit (DNA-Binding Phosphorylation ELISA) - LS-F907 from LifeSpan Bioscience

Category ELISA Kit
Prices $750.00
Sizes 1 plate

Mouse/Human/Rat Phospho-SMAD1 (Ser465) ELISA Kit (Cell-Based Phosphorylation ELISA) - LS-F1056 from LifeSpan Bioscience

Category ELISA Kit
Prices $550.00
Sizes 1 plate

Mouse/Human Phospho-SMAD1 (Ser187) ELISA Kit (Cell-Based Phosphorylation ELISA) - LS-F1372 from LifeSpan Bioscience

Category ELISA Kit
Prices $550.00
Sizes 1 plate

Mouse/Human/Rat SMAD1 ELISA Kit (DNA-Binding ELISA) - LS-F843 from LifeSpan Bioscience

Category ELISA Kit
Prices $700.00
Sizes 1 plate

Mouse/Human/Rat Phospho-SMAD1 (Ser465) ELISA Kit (DNA-Binding Phosphorylation ELISA) - LS-F925 from LifeSpan Bioscience

Category ELISA Kit
Prices $750.00
Sizes 1 plate

Human SMAD1 ELISA Kit (Sandwich ELISA) - LS-F12613 from LifeSpan Bioscience

Category ELISA Kit
Prices $600.00
Sizes 1 plate
Sensitivity 1.56
Range 1.56 - 100 ng/ml

Mothers Against Decapentaplegic Homolog 1 (Smad1), Human, ELISA Kit - SEA647Hu from ARP American Research Products

Category ELISA Kit
Prices $585.00
Sizes 96 t
Sensitivity 0.33ng/mL
Range 0.78 -50ng/mL

Mothers Against Decapentaplegic Homolog 1, Human, ELISA Kit - E01S0269 from ARP American Research Products

Category ELISA Kit
Prices $535.00, $735.00
Sizes 48 t, 96 t
Sensitivity 0.1ng/ml
Range 1.0-25 ng/mL,

Mothers Against Decapentaplegic Homolog 1, Smad1/MADH1, Human, ELISA Kit - E-EL-H0932 from ARP American Research Products

Category ELISA Kit
Prices $522.00
Sizes 96 t
Sensitivity 0.469
Range 0.781-50ng/mL

Mothers Against Decapentaplegic Homolog 1, Smad1, Mouse, ELISA Kit - E-EL-M1062 from ARP American Research Products

Category ELISA Kit
Prices $585.00
Sizes 96 t

Mothers Against Decapentaplegic Homolog 1, Smad1, Rat, ELISA Kit - E-EL-R0637 from ARP American Research Products

Category ELISA Kit
Prices $585.00
Sizes 96 t
Sensitivity 0.188
Range 0.313-20ng/mL

Mothers Against Decapentaplegic Homolog 1, Smad1/MADH1, Rabbit, ELISA Kit - E-EL-RB0675 from ARP American Research Products

Category ELISA Kit
Prices $585.00
Sizes 96 t
Sensitivity 0.188
Range 0.313-20ng/mL

Mothers Against Decapentaplegic Homolog 1, Smad1/MADH1, Chicken, ELISA Kit - E-EL-Ch1917 from ARP American Research Products

Category ELISA Kit
Prices $522.00
Sizes 96 t

Mothers Against Decapentaplegic Homolog 1, Smad1/MADH1, Porcine, ELISA Kit - E-EL-P2217 from ARP American Research Products

Category ELISA Kit
Prices $585.00
Sizes 96 t

Mothers Against Decapentaplegic Homolog 1, Smad1/MADH1, Sheep, ELISA Kit - E-EL-S0195 from ARP American Research Products

Category ELISA Kit
Prices $648.00
Sizes 96 t

Mothers Against Decapentaplegic Homolog 1, Smad1/MADH1, Monkey, ELISA Kit - E-EL-MK1402 from ARP American Research Products

Category ELISA Kit
Prices $585.00
Sizes 96 t

Human MADH 1 ELISA Kit - HS0012 from NeoScientific

Category ELISA Kit
Prices $495.00
Sizes 96 t

Smad1 Colorimetric Cell-Based ELISA Kit - CB5637 from Assay Biotechnology

Category ELISA Kit
Prices $380.00, $760.00, $1,140.00
Sizes 1 kit, 2 kits, 3 kits
Range > 5000 Cells
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