| Gene Symbol | TDP2 |
|---|---|
| Entrez Gene | 51567 |
| Alt Symbol | AD022, EAP2, EAPII, TTRAP, dJ30M3.3, hTDP2 |
| Species | Human |
| Gene Type | protein-coding |
| Description | tyrosyl-DNA phosphodiesterase 2 |
| Other Description | 5'-Tyr-DNA phosphodiesterase|5'-tyrosyl-DNA phosphodiesterase|ETS1-associated protein 2|ETS1-associated protein II|TRAF and TNF receptor associated protein|TRAF and TNF receptor-associated protein|VPg unlinkase|tyr-DNA phosphodiesterase 2|tyrosyl-RNA phosphodiesterase |
| Swissprots | Q2TBE2 Q5JYM0 O95551 B4DKL8 B4DQ95 Q9NYY9 Q7Z6U5 Q9NUK5 |
| Accessions | AAT09764 EAW55458 EAW55459 O95551 AF201687 AAG35600 AF223469 AAF64144 AJ269473 CAB92966 AJ420495 AK002168 BAA92119 AK296623 BAG59230 AK298699 BAG60857 AK308836 BC017553 AAH17553 BC110375 AAI10376 EU446614 ABZ92143 JN104587 AFK13123 XR_926244 NM_016614 NP_057698 |
| Function | DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue. Hydrolyzes 5'- phosphoglycolates on protruding 5' ends on DNA double-strand breaks (DSBs) due to DNA damage by radiation and free radicals. The 5'-tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DSBs without the need for nuclease activity, creating a 'clean' DSB with 5'-phosphate termini that are ready for ligation. Has preference for single-stranded DNA or duplex DNA with a 4 base pair overhang as substrate. Has also 3'- tyrosyl DNA phosphodiesterase activity, but less efficiently and much slower than TDP1. Constitutes the major if not only 5'- tyrosyl-DNA phosphodiesterase in cells. Also acts as a 5'-tyrosyl- RNA phosphodiesterase following picornavirus infection: its activi |
| Subcellular Location | Nucleus. Nucleus, PML body. Nucleus, nucleolus. Cytoplasm. Note=Localizes to nucleolar cavities following stress; localization to nucleolus is dependent on PML protein. In case of infection by picornavirus, relocalizes to cytoplasmic sites distinct from those containing viral proteins associated with RNA replication or encapsidation. |
| Tissue Specificity | Widely expressed. {ECO:0000269|PubMed:10764746}. |
TDP2 CRISPR/Cas9 KO Plasmid (h) - sc-403902 from Santa Cruz Biotechnology
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TDP2 HDR Plasmid (h) - sc-403902-HDR from Santa Cruz Biotechnology
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TDP2 Double Nickase Plasmid (h) - sc-403902-NIC from Santa Cruz Biotechnology
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TDP2 Double Nickase Plasmid (h2) - sc-403902-NIC-2 from Santa Cruz Biotechnology
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TDP2 CRISPR Activation Plasmid (h) - sc-403902-ACT from Santa Cruz Biotechnology
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TDP2 CRISPR Activation Plasmid (h2) - sc-403902-ACT-2 from Santa Cruz Biotechnology
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TDP2 Lentiviral Activation Particles (h) - sc-403902-LAC from Santa Cruz Biotechnology
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TDP2 Lentiviral Activation Particles (h2) - sc-403902-LAC-2 from Santa Cruz Biotechnology
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TDP2 siRNA (h) - sc-60566 from Santa Cruz Biotechnology
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TDP2 shRNA Plasmid (h) - sc-60566-SH from Santa Cruz Biotechnology
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TDP2 shRNA (h) Lentiviral Particles - sc-60566-V from Santa Cruz Biotechnology
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ETS1 associated protein II RNAi - H00051567-R01 from Novus Biologicals
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CompoZr® Knockout ZFN Kit - CKOZFN21184 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND21184 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR07074 from Sigma-Aldrich
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TDP2 siRNA (Human) - CRH9837 from Cohesion Biosciences
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TDP2 - MBS8213725 from MyBioSource
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shRNA set against Human TDP2 (NM_016614.2) - SHH425300 from Creative biogene
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shRNA set against Human TTRAP(NM_016614.2) - SHL103636 from Creative biogene
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TDP2 shRNA Plasmid (Human) - CHH9837 from Cohesion Biosciences
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TDP2 miRNA 3'UTR clone - MiUTR1H-10867 from Creative biogene
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