| Gene Symbol | CENPE |
|---|---|
| Entrez Gene | 1062 |
| Alt Symbol | CENP-E, KIF10, MCPH13, PPP1R61 |
| Species | Human |
| Gene Type | protein-coding |
| Description | centromere protein E, 312kDa |
| Other Description | Centromere autoantigen E (312kD)|centromere-associated protein E|kinesin family member 10|kinesin-related protein CENPE|protein phosphatase 1, regulatory subunit 61 |
| Swissprots | A6NKY9 Q02224 A8K2U7 Q4LE75 |
| Accessions | CBX47458 EAX06169 EAX06170 EAX06171 Q02224 AA210728 AA210858 AB209996 BAE06078 AK290362 BAF83051 AL596934 AU100312 AW499901 AY429526 BC156501 AAI56502 BQ231878 BQ434767 BQ447425 CD644410 CN418651 Z15005 CAA78727 XM_011531544 XP_011529846 XM_011531545 XP_011529847 XM_011531546 XP_011529848 XM_011531547 XP_011529849 XM_011531548 XP_011529850 XM_011531549 XP_011529851 NM_001286734 NP_001273663 NM_001813 NP_001804 |
| Function | Essential for the maintenance of chromosomal stability through efficient stabilization of microtubule capture at kinetochores. Plays a key role in the movement of chromosomes toward the metaphase plate during mitosis. Is a slow plus end- directed motor whose activity is essential for metaphase chromosome alignment. Couples chromosome position to microtubule depolymerizing activity. The highly processive microtubule- dependent motor activity of CENPE serves to power chromosome congression and provides a flexible, motile tether linking kinetochores to dynamic spindle microtubules. Necessary for the mitotic checkpoint signal at individual kinetochores to prevent aneuploidy due to single chromosome loss. Required for the efficient recruitment of BUBR1, MAD1 and MAD2 to attached and newly unattached kinetochores. Stimulates mammalian BUBR1 kinase activity. Accumulates just before mitosis at the G2 phase of the cell cycle. {ECO:0000269|PubMed:17535814, ECO:0000269|PubMed:7889940}. |
| Subcellular Location | Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Note=Associates with kinetochores during congression (as early as prometaphase), relocates to the spindle midzone at anaphase, and is quantitatively discarded at the end of the cell division. |
CENP-E CRISPR/Cas9 KO Plasmid (h) - sc-403015 from Santa Cruz Biotechnology
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CENP-E HDR Plasmid (h) - sc-403015-HDR from Santa Cruz Biotechnology
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CENP-E Double Nickase Plasmid (h) - sc-403015-NIC from Santa Cruz Biotechnology
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CENP-E Double Nickase Plasmid (h2) - sc-403015-NIC-2 from Santa Cruz Biotechnology
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CENP-E CRISPR Activation Plasmid (h) - sc-403015-ACT from Santa Cruz Biotechnology
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CENP-E CRISPR Activation Plasmid (h2) - sc-403015-ACT-2 from Santa Cruz Biotechnology
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CENP-E Lentiviral Activation Particles (h) - sc-403015-LAC from Santa Cruz Biotechnology
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CENP-E Lentiviral Activation Particles (h2) - sc-403015-LAC-2 from Santa Cruz Biotechnology
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CENP-E siRNA (h) - sc-37561 from Santa Cruz Biotechnology
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CENP-E shRNA Plasmid (h) - sc-37561-SH from Santa Cruz Biotechnology
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CENP-E shRNA (h) Lentiviral Particles - sc-37561-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU137841 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN5941 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND5941 from Sigma-Aldrich
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CENPE siRNA (Human) - CRH0766 from Cohesion Biosciences
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CENP-E - MBS826822 from MyBioSource
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CENPE - MBS8229526 from MyBioSource
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shRNA set against Human CENPE (NM_001813.2) - SHH261617 from Creative biogene
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shRNA set against Human CENPE(NM_001813.2) - SHG167995 from Creative biogene
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Human CENPE siRNA - orb261268 from Biorbyt
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CENPE shRNA Plasmid (Human) - CHH0766 from Cohesion Biosciences
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CENPE miRNA 3'UTR clone - MiUTR1H-02037 from Creative biogene
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