CENPA

Gene Symbol CENPA
Entrez Gene 1058
Alt Symbol CENP-A, CenH3
Species Human
Gene Type protein-coding
Description centromere protein A
Other Description centromere autoantigen A|centromere protein A, 17kDa|centromere-specific histone|histone H3-like centromeric protein A
Swissprots Q53T74 P49450 D6W544 Q9BVW2
Accessions AAB47505 AAX93267 EAX00669 EAX00670 EAX00671 EAX00672 P49450 BC000881 AAH00881 BC002703 AAH02703 BC011013 BT007246 AAP35910 HQ448742 ADQ33220 JF432571 ADZ15788 U14518 AAA57416 NM_001042426 NP_001035891 NM_001809 NP_001800
Function Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division. The CENPA-H4 heterotetramer can bind DNA by itself (in vitro). {ECO:0000269|PubMed:20739937, ECO:0000269|PubMed:21478274}.
Subcellular Location Nucleus. Chromosome, centromere, kinetochore. Note=Localizes exclusively in the kinetochore domain of centromeres. Occupies a compact domain at the inner kinetochore plate stretching across 2 thirds of the length of the constriction but encompassing only one third of the constriction width and height.

Phospho-CENP-A (Ser7) Antibody - 2187 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa cells arrested in S phase or mitosis using Phospho-CENP-A (Ser7) Antibody (upper panel) or CENP-A Antibody #2186 (lower panel). S phase cells were treated for 12 hours with thymidine (2 mM), rinsed three times, released into normal growth medium for 10 hours and then treated an additional 12 hours with thymidine before harvesting. Mitotic cells were treated for 12 hours with thymidine, rinsed three times and then treated for 16 hours with paxitaxol (500 nM final).

CENP-A Antibody - 2186 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of various whole cell lysates using CENP-A Antibody.

CENP-A (H-50) - sc-22787 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
CENP-A (H-50): sc-22787. Western blot analysis of CENP-A expression in non-transfected: sc-110760 (A) and human CENP-A transfected: sc-110471 (B) 293 whole cell lysates.

CENP-A (C-17) - sc-11278 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

CENP-A (A-15) - sc-11277 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-CENPA (phospho S7) antibody - ab62242 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab62242 at 1/500 - 1/1000 dilution staining CENPA in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.

Anti-CENPA antibody - ab65678 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded human skin tissue stained with ab65678 at 4µg/ml. Positively stained squamous epithelial cells are indicated with arrows.

Anti-CENPA antibody - ab62688 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab62688 at 1/500 - 1/1000 dilution staining CENPA in HeLa cells (treated with Forskolin (40nM, 30mins) or untreated) by Immunofluorescence,

Anti-CENPA antibody - ab118588 from Abcam

Prices $376.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Human Colon using ab118588 at a concentration of 2.5 µg/ml.

Anti-CENPA antibody - ab116571 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-CENPA antibody (ab116571) at 1/100 dilution + Jurkat cell line lysate at 35 µg

Anti-CENPA antibody [3-19] - ChIP Grade - ab13939 from Abcam

Prices $403.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 3-19

Anti-CENPA antibody [EP800Y] - Centromere Marker - ab45694 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP800Y
Overlay histogram showing HeLa cells stained with ab45694 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab45694, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-CENPA / CENP-A Antibody (phospho-Ser7) - LS-C6049 from LifeSpan Bioscience

Prices $575.00
Sizes 200 µg
Host Rabbit
Clonality Polyclonal

Anti-CENPA / CENP-A Antibody (aa4-17) - LS-C6047 from LifeSpan Bioscience

Prices $515.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-CENPA / CENP-A Antibody (aa3-19) - LS-C6050 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-CENPA / CENP-A Antibody (phospho-Ser7) - LS-C79056 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-CENPA / CENP-A Antibody - LS-C79054 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-CENPA / CENP-A Antibody (aa11-60) - LS-C119449 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-CENPA / CENP-A Antibody (N-Terminus) - LS-C144676 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-CENPA / CENP-A Antibody (phospho-Ser7) - LS-C198792 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence of HeLa cells, using Centromeric Protein A (Phospho-Ser7) Antibody. The sample on the right was incubated with synthetic peptide.

Anti-CENPA / CENP-A Antibody (aa1-50) - LS-C117855 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC of paraffin-embedded human breast carcinoma tissue, using Centromeric Protein A (Ab-7) Antibody. The picture on the right is treated with the synthesized peptide.

Anti-CENPA / CENP-A Antibody (clone 5A7-2E11, Atto 488) - LS-C227558 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 5A7-2E11

Anti-CENPA / CENP-A Antibody (clone 1D4-1A3) - LS-C196898 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1D4-1A3
Detection limit for recombinant GST tagged CENPA is approximately 0.3 ng/ml as a capture antibody.

Anti-CENPA / CENP-A Antibody (clone 5A7-2E11, Atto 565) - LS-C227561 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 5A7-2E11

Anti-CENPA / CENP-A Antibody (clone 5A7-2E11, Atto 390) - LS-C227559 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 5A7-2E11
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