PCGF2

Gene Symbol PCGF2
Entrez Gene 7703
Alt Symbol MEL-18, RNF110, ZNF144
Species Human
Gene Type protein-coding
Description polycomb group ring finger 2
Other Description DNA-binding protein Mel-18|polycomb group RING finger protein 2|ring finger protein 110|zinc finger protein 144
Swissprots P35227 A6NGD8
Accessions CAI84554 EAW60527 EAW60528 EAW60529 EAW60530 P35227 AM392769 CAL37647 AU123693 BC004858 AAH04858 BC024255 AAH24255 BX647429 CA421781 D13969 BAA03074 XM_005257640 XP_005257697 XM_005257641 XP_005257698 XM_005257642 XP_005257699 XM_006725342 XP_006725405 XM_006725344 XP_006725407 XM_006725345 XP_006725408 NM_007144 NP_009075
Function Transcriptional repressor. Binds specifically to the DNA sequence 5'-GACTNGACT-3'. Has tumor suppressor activity. May play a role in control of cell proliferation and/or neural cell development. Regulates proliferation of early T progenitor cells by maintaining expression of HES1. Also plays a role in antero- posterior specification of the axial skeleton and negative regulation of the self-renewal activity of hematopoietic stem cells. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Is not functionally redundant with BMI1; unlike BMI1 does not stimulate the E3 ubiquitin-protein ligase activity in a reconstituted PRC1-like complex (By similari
Subcellular Location Nucleus {ECO:0000269|PubMed:21282530}.
Tissue Specificity Detected in all tissues examined with high expression found in placenta lung and kidney and low expression, in liver, pancreas and skeletal muscle.
Top Pathways Signaling pathways regulating pluripotency of stem cells

Mel-18 (B-8) - sc-390868 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Mel-18 (B-8): sc-390868. Western blot analysis of Mel-18 expression in human liver tissue extract.

Mel-18 (C-20) - sc-8905 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Mel-18 (C-20): sc-8905. Western blot analysis of human recombinant Mel-18 fusion protein.

Mel-18 (H-115) - sc-10744 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Mel-18 (H-115): sc-10744. Western blot analysis of Mel-18 expression in HeLa (A) and Jurkat (B) whole cell lysates.

Mel-18 (N-17) - sc-8904 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Mel-18 (N-17): sc-8904. Western blot analysis of Mel-18 expression in human liver tissue extract.

Mel-18 (9HL1) - sc-130415 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Mel-18 (9HL1): sc-130415. Western blot analysis of Mel-18 expression in human liver tissue extract.

Anti-Mel18 antibody - ab77255 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Anti-Mel18 antibody (ab77255) at 5 µg/ml + immunogen at 0.2 µg/mlSecondary Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilutiondeveloped using the ECL technique

Anti-Mel18 antibody - ab16651 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab16651 stained human MCF7 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab16651, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HepG2 cells.

Anti-Mel18 antibody - ChIP Grade - ab5267 from Abcam

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-Mel18 antibody - N-terminal - ab166656 from Abcam

Host Rabbit
Clonality Polyclonal
Anti-Mel18 antibody - N-terminal (ab166656) at 1/1000 dilution + Mouse brain total cell extract

Anti-PCGF2 / MEL-18 Antibody - LS-C125928 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PCGF2 / MEL-18 Antibody (aa121-150, Azide-free, HRP) - LS-C223596 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PCGF2 / MEL-18 Antibody (aa121-150, PE) - LS-C223597 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PCGF2 / MEL-18 Antibody (aa121-150, AP) - LS-C226954 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PCGF2 / MEL-18 Antibody (aa121-150, APC) - LS-C226955 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PCGF2 / MEL-18 Antibody (aa121-150, Biotin) - LS-C226956 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PCGF2 / MEL-18 Antibody (aa121-150, FITC) - LS-C226957 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PCGF2 / MEL-18 Antibody (aa120-150) - LS-C323853 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PCGF2 / MEL-18 Antibody (aa332-344) - LS-C34654 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
PCGF2 antibody staining (1 ug/ml) of Human Lung lysate (RIPA buffer, 30g total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Anti-PCGF2 / MEL-18 Antibody (aa121-150) - LS-C163443 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
PCGF2 Antibody western blot of A549 cell line lysates (35 ug/lane). The PCGF2 antibody detected the PCGF2 protein (arrow).

Anti-PCGF2 / MEL-18 Antibody (clone 4D6) - LS-C198481 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 4D6
PCGF2 monoclonal antibody (M03), clone 4D6. Western Blot analysis of PCGF2 expression in human liver.

Anti-PCGF2 / MEL-18 Antibody (aa125-175) - LS-C290246 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human Mel-18 by Western Blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Mel-18 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

MEL-18 Antibody - 21650002 from Novus Biologicals

Prices $329.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

MEL-18 Antibody (4D6) - H00007703-M03 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 4D6
Western Blot: MEL-18 Antibody (4D6) [H00007703-M03] - Analysis of PCGF2 expression in human liver.

MEL-18 Antibody (4C10) - H00007703-M04 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 4C10
Western Blot: MEL-18 Antibody (4C10) [H00007703-M04] - Analysis of PCGF2 expression in RIN-m5F.

MEL-18 Antibody - NBP2-04032 from Novus Biologicals

Prices $369.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: MEL-18 Antibody [NBP2-04032] - Whole cell lysate from HeLa (15 and 50 ug), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibody used at 0.1 ug/ml.
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