Gene Symbol | PNKP |
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Entrez Gene | 11284 |
Alt Symbol | AOA4, EIEE10, MCSZ, PNK |
Species | Human |
Gene Type | protein-coding |
Description | polynucleotide kinase 3'-phosphatase |
Other Description | DNA 5'-kinase/3'-phosphatase|Homo sapiens polynucleotide kinase 3'-phosphatase (PNKP)|bifunctional polynucleotide phosphatase/kinase |
Swissprots | Q9BUL2 Q96T60 Q9UKU8 Q9P1V2 Q9UNI0 Q9UNF8 |
Accessions | AAF44716 AAK57340 AAM82170 EAW52565 EAW52566 EAW52567 Q96T60 AA640353 AF120499 AAD47379 AF125807 AAD50639 AF126486 AAD51135 BC002519 AAH02519 BC009339 BC013034 BC033822 AAH33822 BU541073 DQ890806 ABM81732 DQ893961 ABM84887 NM_007254 NP_009185 |
Function | Plays a key role in the repair of DNA damage, functioning as part of both the non-homologous end-joining (NHEJ) and base excision repair (BER) pathways. Through its two catalytic activities, PNK ensures that DNA termini are compatible with extension and ligation by either removing 3'-phosphates from, or by phosphorylating 5'-hydroxyl groups on, the ribose sugar of the DNA backbone. {ECO:0000269|PubMed:10446192}. |
Subcellular Location | Nucleus {ECO:0000269|PubMed:10446193}. |
Tissue Specificity | Expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine (PubMed:10446192). Expressed in higher amount in pancreas, heart and kidney and at lower levels in brain, lung and liver (PubMed:10446193). {ECO:0000269|PubMed:10446192, ECO:0000269|PubMed:10446193}. |
PNK CRISPR/Cas9 KO Plasmid (h) - sc-404266 from Santa Cruz Biotechnology
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PNK HDR Plasmid (h) - sc-404266-HDR from Santa Cruz Biotechnology
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PNK Double Nickase Plasmid (h) - sc-404266-NIC from Santa Cruz Biotechnology
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PNK Double Nickase Plasmid (h2) - sc-404266-NIC-2 from Santa Cruz Biotechnology
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PNK CRISPR Activation Plasmid (h) - sc-404266-ACT from Santa Cruz Biotechnology
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PNK CRISPR Activation Plasmid (h2) - sc-404266-ACT-2 from Santa Cruz Biotechnology
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PNK Lentiviral Activation Particles (h) - sc-404266-LAC from Santa Cruz Biotechnology
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PNK Lentiviral Activation Particles (h2) - sc-404266-LAC-2 from Santa Cruz Biotechnology
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PNK siRNA (h) - sc-44826 from Santa Cruz Biotechnology
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PNK shRNA Plasmid (h) - sc-44826-SH from Santa Cruz Biotechnology
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PNK shRNA (h) Lentiviral Particles - sc-44826-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU132321 from Sigma-Aldrich
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MISSION® Human Phosphatase - SIHP0428 from Sigma-Aldrich
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MISSION® Human Phosphatase - SIHP0427 from Sigma-Aldrich
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MISSION® Human Phosphatase - SIHP0429 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN17045 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND17045 from Sigma-Aldrich
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PNKP siRNA (Human) - CRH7739 from Cohesion Biosciences
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PNK RNAi - H00011284-R04 from Novus Biologicals
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shRNA set against Human PNKP (NM_007254.3) - SHH378568 from Creative biogene
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PNKP shRNA Plasmid (Human) - CHH7739 from Cohesion Biosciences
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PNKP miRNA 3'UTR clone - MiUTR3H-07833 from Creative biogene
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