TRIM25

Gene Symbol TRIM25
Entrez Gene 7706
Alt Symbol EFP, RNF147, Z147, ZNF147
Species Human
Gene Type protein-coding
Description tripartite motif containing 25
Other Description E3 ubiquitin/ISG15 ligase TRIM25|RING finger protein 147|estrogen-responsive finger protein|tripartite motif protein TRIM25|tripartite motif-containing 25|tripartite motif-containing protein 25|ubiquitin/ISG15-conjugating enzyme TRIM25|zinc finger protein 147 (estrogen-responsive finger protein)|zinc finger protein-147
Swissprots Q14258
Accessions EAW94523 EAW94524 EAW94525 Q14258 AB208994 BAD92231 AK024597 AK310720 BC013752 BC016924 AAH16924 BC038247 BC042541 AAH42541 BC053605 BC106889 BC106890 BC114339 BM146967 BX538220 CF121587 D21205 BAA04747 NM_005082 NP_005073
Function Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs. {ECO:0000269|PubMed:16352599, ECO:0000269|PubMed:17069755, ECO:0000269|PubMed:17392790}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:17392790}. Note=Colocalized with DDX58 at cytoplasmic perinuclear bodies.
Tissue Specificity Ubiquitous. {ECO:0000269|PubMed:15130519}.
Top Pathways RIG-I-like receptor signaling pathway, Influenza A

TRIM25 (D9T7G) Rabbit mAb - 13773 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D9T7G
Western blot analysis of extracts from various cell lines using TRIM25 (D9T7G) Rabbit mAb.

TRIM25 Antibody - 12315 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

EFP (2) - sc-135893 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
EAAT2 (20): sc-135892. Western blot analysis of EAAT2 expression in rat cerebrum tissue extract.

EFP (D-8) - sc-398817 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
EFP (D-8): sc-398817. Western blot analysis of EFP expression in MCF7 (A), HeLa (B) and C32 (C) nuclear extracts and HeLa whole cell lysate (D).

EFP (E-12) - sc-271254 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
EFP (E-12): sc-271254. Immunoperoxidase staining of formalin fixed, paraffin-embedded human oral mucosa tissue showing cytoplasmic staining of subset of epithelial cells.

EFP (E-4) - sc-166926 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
EFP (E-4): sc-166926. Western blot analysis of EFP expression in non-transfected 293T: sc-117752 (A), human EFP transfected 293T: sc-115553 (B) and HeLa (C) whole cell lysates.

EFP (H-300) - sc-22832 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
EFP (H-300): sc-22832. Western blot analysis of EFP expression in HeLa (A) and C32 (B) nuclear extracts.

EFP (S-16) - sc-30890 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
EFP (S-16): sc-30890. Western blot analysis of EFP expression in non-transfected: sc-117752 (A) and human EFP transfected: sc-113410 (B) 293T whole cell lysates.

Anti-TRIM25 antibody - ab115737 from Abcam

Prices $384.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-TRIM25 antibody (ab115737) at 1 µg/mlLane 1 : HeLa cell lysate Lane 2 : HeLa cell lysate with blocking peptide

Anti-TRIM25 antibody - ab203210 from Abcam

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed and paraffin embedded Human breast carcinoma tissue labeling TRIM25 using ab203210 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Anti-TRIM25 antibody - ab86365 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-TRIM25 antibody (ab86365) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgLane 4 : 293T whole cell lysate at 50 µg

Anti-TRIM25 antibody - ab88669 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-TRIM25 antibody (ab88669) at 1 µg/ml + Hela nuclear extract lysate at 50 µg

Anti-TRIM25 antibody - ab126975 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-TRIM25 antibody (ab126975) at 1/1000 dilution + A549 whole cell lysate at 30 µg/mlPerformed under reducing conditions.

Anti-TRIM25 antibody - ab153743 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-TRIM25 antibody (ab153743) at 1/3000 dilution + A431 whole cell lysate at 30 µg

Anti-TRIM25 antibody - ab61305 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Anti-TRIM25 antibody (ab61305) at 1 µg/ml + HeLa nuclear extract at 25 µg

Anti-TRIM25 antibody - ab65223 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-TRIM25 antibody (ab65223) at 1/500 dilutionLane 1 : K562 cell extractLane 2 : K562 cell extract with immunising peptide at 10 µgLysates/proteins at 30 µg per lane.

Anti-TRIM25 antibody [EPR7315] - ab167154 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7315
All lanes : Anti-TRIM25 antibody [EPR7315] (ab167154) at 1/10000 dilutionLane 1 : HeLa cell lysateLane 2 : K562 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labeled goat anti-rabbit at 1/2000 dilution

Anti-TRIM25 antibody [EPR7315] (Alexa Fluor® 488) - ab200781 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7315
ab200781 staining TRIM25 in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab200781 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 2µg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-TRIM25 antibody [EPR7315] (Alexa Fluor® 647) - ab200786 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7315
ab200786 staining TRIM25 in HeLa cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab200786 at 1/200 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 2µg/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-TRIM25 Antibody - LS-C155453 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
IHC of paraffin-embedded Colon ca, using TRIM25 antibody at 1:500 dilution.

Anti-TRIM25 Antibody - LS-C334403 from LifeSpan Bioscience

Prices $305.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines, using TRIM25 antibody.

Anti-TRIM25 Antibody (clone 5C3) IHC-plus™ - LS-B12178 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 5C3
Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)

Anti-TRIM25 Antibody (C-Terminus) IHC-plus™ - LS-B2701 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-TRIM25 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2701 concentration 10 ug/ml.

Anti-TRIM25 Antibody (aa251-300) - LS-C116770 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
TRIM25 antibody LS-C116770 Western blot of RPMI-8226 cell lysate.

Anti-TRIM25 Antibody (aa359-408) - LS-C116771 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
TRIM25 antibody LS-C116771 Western blot of Fetal Liver lysate.
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