AURKA

Gene Symbol AURKA
Entrez Gene 6790
Alt Symbol AIK, ARK1, AURA, AURORA2, BTAK, PPP1R47, STK15, STK6, STK7
Species Human
Gene Type protein-coding
Description aurora kinase A
Other Description ARK-1|Aurora-A kinase|IPL1-related kinase|aurora 2|aurora-related kinase 1|aurora/IPL1-like kinase|aurora/IPL1-related kinase 1|breast tumor-amplified kinase|breast-tumor-amplified kinase|hARK1|protein phosphatase 1, regulatory subunit 47|serine/threonine kinase 6|serine/threonine protein kinase 15|serine/threonine-protein kinase 15|serine/threonine-protein kinase 6|serine/threonine-protein kinase aurora-A
Swissprots Q9UPG5 Q9BQD6 O14965 O75873 O60445 E1P5F9
Accessions AAC23448 AAF29508 EAW75550 EAW75551 EAW75552 EAW75553 EAW75554 EAW75555 EAW75556 EAW75557 EAW75558 EAW75559 EAW75560 EAW75561 EAW75562 O14965 AF008551 AAC12708 AF011468 AAC63902 AK301769 BAG63228 AK312776 BAG35640 AL711075 AM392948 CAL37826 BC001280 AAH01280 BC002499 AAH02499 BC006423 AAH06423 BC027464 AAH27464 BF029182 BM458030 BQ435059 BU171303 BU542363 CB113289 CB138172 D84212 BAA23592 DQ891270 ABM82196 DQ894458 ABM85384 XM_005260534 XP_005260591 XM_006723872 XP_006723935 XM_011529021 XP_011527323 NM_003600 NP_003591 NM_198433 NP_940835 NM_198434 NP_940836 NM_198435 NP_940837 NM_198436 NP_940838 NM_198437 NP_940839
Function Mitotic serine/threonine kinases that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis. Required for initial activation of CDK1 at centrosomes. Phosphorylates numerous target proteins, including ARHGEF2, BORA, BRCA1, CDC25B, DLGP5, HDAC6, KIF2A, LATS2, NDEL1, PARD3, PPP1R2, PLK1, RASSF1, TACC3, p53/TP53 and TPX2. Regulates KIF2A tubulin depolymerase activity. Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization. Also acts as a key regulatory component of the p53/TP53 pathway, and particularly the checkpoint-response pathways critical for oncogenic transformation of cel
Subcellular Location Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Note=Detected at the neurite hillock in developing neurons (By similarity). Localizes at the centrosome in mitotic cells from early prophase until telophase, but also localizes to the spindle pole MTs from prophase to anaphase. Colocalized with SIRT2 at centrosome. Moves to the midbody during both telophase and cytokinesis. Associates with both the pericentriolar material (PCM) and centrioles. {ECO:0000250}.
Tissue Specificity Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.
Top Pathways Oocyte meiosis

Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) (D13A11) XP ® Rabbit mAb (Alexa Fluor ® 555 Conjugate) - 13464 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D13A11
Confocal immunofluorescent analysis of HeLa cells using Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) (D13A11) XP ® Rabbit mAb (Alexa Fluor ® 555 Conjugate) (red). Actin filaments were labeled with Alexa Fluor ® 488 phalloidin #8878 (green). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).

Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) (D13A11) XP ® Rabbit mAb (Alexa Fluor ® 488 Conjugate) - 8525 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D13A11
Two-color flow cytometric analysis of asynchronous Jurkat cells using Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) (D13A11) XP ® Rabbit mAb (Alexa Fluor ® 488 Conjugate) and Phospho-Histone H3 (Ser10) (D2C8) XP ® Rabbit mAb (Alexa Fluor ® 647 Conjugate) #3458. Cells represented in green (gated) are mitotic and positive for phospho-Aurora and phospho-histone H3, while cells represented in blue are non-mitotic.

Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) (D13A11) XP ® Rabbit mAb (Biotinylated) - 2995 from Cell Signaling Technology

Host Rabbit
Clonality Monoclonal
Clone D13A11

Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) (D13A11) XP ® Rabbit mAb - 2914 from Cell Signaling Technology

Prices $119.00, $318.00
Sizes 20 µl (2 western blots), 100 µl (20 western blots)
Host Rabbit
Clonality Monoclonal
Clone D13A11
Western blot analysis of extracts from HeLa, L929 and C6 cells, treated with 4 mM hydroxyurea for 20 hours to induce G1/S phase or treated with 100 nM paclitaxel or 100 ng/ml nocodazole for 20 hours to induce G2/M phase, using Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) (D11A13) XP ® Rabbit mAb (upper) or Aurora B/AIM1 Antibody #3094 (lower).

Phospho-Aurora A (Thr288) (C39D8) Rabbit mAb - 3079 from Cell Signaling Technology

Prices $109.00, $297.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C39D8
Western blot analysis of extracts from hydroxyurea or nocodazole treated HeLa and HT29 cells using Phospho-Aurora A (Thr288) (C39D8) Rabbit mAb.

Aurora A (D3E4Q) Rabbit mAb - 14475 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D3E4Q
Western blot analysis of extracts from HT-29 cells, untreated (-) or synchronized in mitosis by treatment with thymidine (2 mM, 17 hr; +) and Nocodazole #2190 (100 ng/ml, 24 hr; +), using Aurora A (D3E4Q) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Aurora A/AIK Antibody - 3092 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa, HepG2 and COS cells, using Aurora A/AIK Antibody.

Aurora A (1F8) Mouse mAb - 12100 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 1F8
Western blot analysis of extracts from various cell lines using Aurora A (1F8) Mouse mAb.

Aurora A/AIK (1G4) Rabbit mAb - 4718 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 1G4
Western blot analysis of extracts from HeLa, 293 and COS cells, using Aurora A/AIK (1G4) Rabbit mAb.

Phospho-Aurora A/AIK (Thr288) Antibody - 3091 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) Antibody - 3068 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

ARK-1 (A-11) - sc-514374 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
ARK-1 (A-11): sc-514374. Western blot analysis of ARK-1 expression in HeLa whole cell lysate.

ARK-1 (C-1) - sc-398814 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
ARK-1 (C-1): sc-398814. Western blot analysis of ARK-1 expression in non-transfected 293: sc-110760 (A), human ARK-1 transfected 293: sc-128042 (B), HeLa (C) and BT-20 (D) whole cell lysates.

ARK-1 (H-130) - sc-25425 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
ARK-1 (H-130): sc-25425. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

ARK-1 (35C1) - sc-56881 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
ARK-1 (35C1): sc-56881. Western blot analysis of ARK-1 expression in HeLa (A), Daudi (B) and NTERA-2 cl.D1 (C) whole cell lysates.

ARK-1 (N-20) - sc-14318 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
ARK-1 (N-20): sc-14318. Western blot analysis of ARK-1 expression in HeLa whole cell lysate.

ARK-1 (T-16) - sc-14321 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
ARK-1 (T-16): sc-14321. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining.

p-ARK-1 (Thr 288) - sc-109910 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal

p-ARK-1 (95.Thr 288) - sc-293126 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of ARK-1 phosphorylation in untreated (A,D), nocodazole treated (B,E) and nocodazole and lambda protein phosphatase (sc-200312A) treated (C,F) HeLa whole cell lysates. Antibodies tested include p-ARK-1 (95.Thr 288): sc-293126 (A,B,C) and ARK-1 (H-130): sc-25425 (D,E,F).

Anti-Aurora A + B antibody [3H1] - ab190374 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3H1
All lanes : Anti-Aurora A + B antibody [3H1] (ab190374) at 1/1000 dilutionLane 1 : HeLa cells treated with 100ng/ml nocodazole for 18 hoursLane 2 : Recombinant Aurora ALane 3 : Recombinant Aurora BLane 4 : Recombinant Aurora C

Anti-Aurora A (phospho S342) antibody - ab111410 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using 1/50 ab111410. The picture on the right is treated with the phopspho peptide.

Anti-Aurora A (phospho T288) antibody - ab18318 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-Aurora A (phospho T288) antibody - ab195748 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Aurora A (phospho T288) antibody (ab195748) at 1/500 dilutionLane 1 : Serum treated 293 whole cell lysates with antigen-specific peptideLane 2 : Serum treated 293 whole cell lysatesLysates/proteins at 12.5 µg per lane.

Anti-Aurora A (phospho T288) antibody - ab118634 from Abcam

Host Rabbit
Clonality Polyclonal
ab118634 at 1/50 dilution staining Aurora A (phospho T288) in Formalin-Fixed, Paraffin-Embedded Human Testis tissue by Immunohistochemistryfollowed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-Aurora A (phospho T288) antibody - ab83968 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab83968, at a dilution of 1/250, staining Aurora A in NBF fixed asynchronous HeLa cells by Immunocytochemistry/Immunofluorescence. Detection: Anti-rabbit IgG-FITC conjugated antibody used at a dilution of 1/100.
Search more