PHF2

Gene Symbol PHF2
Entrez Gene 5253
Alt Symbol CENP-35, GRC5, JHDM1E
Species Human
Gene Type protein-coding
Description PHD finger protein 2
Other Description centromere protein 35|jumonji C domain-containing histone demethylase 1E|lysine-specific demethylase PHF2
Swissprots Q4VXG0 Q9Y6N4 O75151 Q8N3K2
Accessions EAW62867 EAW62868 EAW62869 EAW62870 EAW62871 O75151 AB014562 BAA31637 AF043725 AAD21791 AK294861 BAG57965 AL834263 CAD38938 BC028051 AAH28051 BC050112 BC064613 AAH64613 BC152437 BC172253 AAI72253 BC172560 AAI72560 BQ716020 BU507256 CB990295 XM_005252051 XP_005252108 XM_006717143 XP_006717206 XM_011518784 XP_011517086 NM_005392 NP_005383 NM_024517
Function Lysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B. Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3 (H3K4me3) at rDNA promoters and promotes expression of rDNA. {ECO:0000269|PubMed:20129925, ECO:0000269|PubMed:21167174, ECO:0000269|PubMed:21532585}.
Subcellular Location Nucleus, nucleolus {ECO:0000269|PubMed:20129925}. Chromosome, centromere, kinetochore {ECO:0000269|PubMed:20813266}.
Tissue Specificity Widely expressed, including in liver (at protein level). {ECO:0000269|PubMed:21532585}.

PHF2 (D45A2) Rabbit mAb - 3497 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D45A2
Western blot analysis of extracts from various cell lines using PHF2 (D45A2) Rabbit mAb.

PHF2 (C-17) - sc-324199 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PHF2 (C-17): sc-324199. Immunofluorescence staining of formalin-fixed HeLa cells showing nuclear localization. Kindly provided by Yang Xiang, Ph.D., Division of Newborn Medicine, Boston Childrens Hospital, Cell Biology Department, Harvard Medical School.

PHF2 (I-13) - sc-101864 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
PHF2 (I-13): sc-101864. Western blot analysis of PHF2 expression in non-transfected: sc-117752 (A) and mouse PHF2 transfected: sc-122538 (B) 293T whole cell lysates.

Anti-GRC5 / PHF2 antibody - ab124434 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-GRC5 / PHF2 antibody (ab124434) at 1 µg/mlLane 1 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lane 2 : Liver (Mouse) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-GRC5 / PHF2 antibody - ab55619 from Abcam

Host Mouse
Clonality Monoclonal
Anti-GRC5 / PHF2 antibody (ab55619) at 5 µg/ml + 0.2 µg/lane of partial recombinant GRC5 / PHF2 protein with a tagSecondaryGoat anti mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

Anti-GRC5 / PHF2 antibody - ab123507 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-GRC5 / PHF2 antibody (ab123507) at 0.1 µg/mlLane 1 : Whole cell lysate from HeLa at 15 µgLane 2 : Whole cell lysate from HeLa at 50 µgLane 3 : Whole cell lysate from 293T at 50 µgLane 4 : Whole cell lysate from Jurkat at 50 µgdeveloped using the ECL technique

Anti-GRC5 / PHF2 antibody - ab90880 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-GRC5 / PHF2 antibody - ab1138 from Abcam

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-GRC5 / PHF2 antibody [5D4] - ab154983 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5D4

Anti-GRC5 / PHF2 antibody - Carboxyterminal end - ab65771 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-GRC5 / PHF2 antibody - Carboxyterminal end (ab65771) at 1/1000 dilution + Nuclear extracts of HeLa cells.

Anti-PHF2 Antibody (aa70-82) - LS-C40576 from LifeSpan Bioscience

Prices $275.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PHF2 Antibody (clone 3E7) - LS-C197892 from LifeSpan Bioscience

Prices $355.00
Sizes 200 µl
Host Mouse
Clonality Monoclonal
Clone 3E7

Anti-PHF2 Antibody (aa936-985) - LS-C109733 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
PHF2 antibody LS-C109733 Western blot of Jurkat lysate.

Anti-PHF2 Antibody (aa650-700) - LS-C290132 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human PHF2 by Western Blot. Samples : Whole cell lysate from HeLa (15 and 50 ug), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies : Affinity purified rabbit anti-PHF2 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Anti-PHF2 Antibody IHC-plus™ - LS-B172 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-PHF2 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B172 concentration 20 ug/ml.

PHF2 Antibody - H00005253-A01 from Novus Biologicals

Prices $269.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal

PHF2 Antibody (3E7) - H00005253-M02A from Novus Biologicals

Prices $349.00
Sizes 200 µl
Host Mouse
Clonality Monoclonal
Clone 3E7

GRC5/PHF2 Polyclonal Antibody - bs-11659R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

GRC5/PHF2 Polyclonal Antibody, ALEXA FLUOR® 350 Conjugated - bs-11659R-A350 from Bioss

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

GRC5/PHF2 Polyclonal Antibody, ALEXA FLUOR® 488 Conjugated - bs-11659R-A488 from Bioss

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

GRC5/PHF2 Polyclonal Antibody, ALEXA FLUOR® 555 Conjugated - bs-11659R-A555 from Bioss

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

GRC5/PHF2 Polyclonal Antibody, ALEXA FLUOR® 647 Conjugated - bs-11659R-A647 from Bioss

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

GRC5/PHF2 Polyclonal Antibody, Cy3 Conjugated - bs-11659R-Cy3 from Bioss

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

GRC5/PHF2 Polyclonal Antibody, Cy5 Conjugated - bs-11659R-Cy5 from Bioss

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

GRC5/PHF2 Polyclonal Antibody, Cy5.5 Conjugated - bs-11659R-Cy5.5 from Bioss

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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