Gene Symbol | PHF2 |
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Entrez Gene | 5253 |
Alt Symbol | CENP-35, GRC5, JHDM1E |
Species | Human |
Gene Type | protein-coding |
Description | PHD finger protein 2 |
Other Description | centromere protein 35|jumonji C domain-containing histone demethylase 1E|lysine-specific demethylase PHF2 |
Swissprots | Q4VXG0 Q9Y6N4 O75151 Q8N3K2 |
Accessions | EAW62867 EAW62868 EAW62869 EAW62870 EAW62871 O75151 AB014562 BAA31637 AF043725 AAD21791 AK294861 BAG57965 AL834263 CAD38938 BC028051 AAH28051 BC050112 BC064613 AAH64613 BC152437 BC172253 AAI72253 BC172560 AAI72560 BQ716020 BU507256 CB990295 XM_005252051 XP_005252108 XM_006717143 XP_006717206 XM_011518784 XP_011517086 NM_005392 NP_005383 NM_024517 |
Function | Lysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B. Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3 (H3K4me3) at rDNA promoters and promotes expression of rDNA. {ECO:0000269|PubMed:20129925, ECO:0000269|PubMed:21167174, ECO:0000269|PubMed:21532585}. |
Subcellular Location | Nucleus, nucleolus {ECO:0000269|PubMed:20129925}. Chromosome, centromere, kinetochore {ECO:0000269|PubMed:20813266}. |
Tissue Specificity | Widely expressed, including in liver (at protein level). {ECO:0000269|PubMed:21532585}. |
PHF2 CRISPR/Cas9 KO Plasmid (h) - sc-407404 from Santa Cruz Biotechnology
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PHF2 HDR Plasmid (h) - sc-407404-HDR from Santa Cruz Biotechnology
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PHF2 Double Nickase Plasmid (h) - sc-407404-NIC from Santa Cruz Biotechnology
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PHF2 Double Nickase Plasmid (h2) - sc-407404-NIC-2 from Santa Cruz Biotechnology
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PHF2 CRISPR Activation Plasmid (h) - sc-407404-ACT from Santa Cruz Biotechnology
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PHF2 CRISPR Activation Plasmid (h2) - sc-407404-ACT-2 from Santa Cruz Biotechnology
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PHF2 Lentiviral Activation Particles (h) - sc-407404-LAC from Santa Cruz Biotechnology
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PHF2 Lentiviral Activation Particles (h2) - sc-407404-LAC-2 from Santa Cruz Biotechnology
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PHF2 siRNA (h) - sc-92588 from Santa Cruz Biotechnology
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PHF2 shRNA Plasmid (h) - sc-92588-SH from Santa Cruz Biotechnology
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PHF2 shRNA (h) Lentiviral Particles - sc-92588-V from Santa Cruz Biotechnology
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CompoZr® Knockout ZFN Kit - CKOZFN16724 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND16724 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR10548 from Sigma-Aldrich
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PHF2 siRNA (Human) - CRH3507 from Cohesion Biosciences
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shRNA set against Human PHF2(NM_005392.3) - SHR111160 from Creative biogene
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Human PHF2 siRNA - orb264009 from Biorbyt
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PHF2 shRNA Plasmid (Human) - CHH3507 from Cohesion Biosciences
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PHF2 miRNA 3'UTR clone - MiUTR1H-07856 from Creative biogene
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