CENPF

Gene Symbol CENPF
Entrez Gene 1063
Alt Symbol CENF, PRO1779, hcp-1
Species Human
Gene Type protein-coding
Description centromere protein F, 350/400kDa
Other Description AH antigen|CENP-F kinetochore protein|cell-cycle-dependent 350K nuclear protein|centromere protein F|centromere protein F, 350/400kDa (mitosin)|centromere protein F, 350/400ka (mitosin)|kinetochore protein CENPF|mitosin
Swissprots Q5VVM7 Q13171 P49454 Q13246
Accessions CBX47616 EAW93350 P49454 AK307690 BC172232 AAI72232 BG680819 BQ774943 BU155312 BU662228 CD654009 U19769 AAA82889 U25725 AAA86889 U30872 AAA82935 XM_011509082 XP_011507384 XM_011509083 XP_011507385 NM_016343 NP_057427
Function Required for kinetochore function and chromosome segregation in mitosis. Required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1. Regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation. May play a regulatory or permissive role in the normal embryonic cardiomyocyte cell cycle and in promoting continued mitosis in transformed, abnormally dividing neonatal cardiomyocytes. Interaction with RB directs embryonic stem cells toward a cardiac lineage. Involved in the regulation of DNA synthesis and hence cell cycle progression, via its C-terminus. Has a potential role regulating skeletal myogenesis and in cell differentiation in embryogenesis. Involved in dendritic cell regulation of T-cell immunity ag
Subcellular Location Cytoplasm, perinuclear region. Nucleus matrix. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Note=Relocalizes to the kinetochore/centromere (coronal surface of the outer plate) and the spindle during mitosis. Observed in nucleus during interphase but not in the nucleolus. At metaphase becomes localized to areas including kinetochore and mitotic apparatus as well as cytoplasm. By telophase, is concentrated within the intracellular bridge at either side of the mid-body.

CENP-F (C-19) - sc-11295 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

CENP-F (H-260) - sc-22791 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
CENP-F (H-260): sc-22791. Immunoperoxidase staining of formalin fixed, paraffin-embedded human lateral ventricle tissue showing nuclear staining of neuronal and non-neuronal cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

CENP-F (N-18) - sc-11292 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
CENP-F (N-18): sc-11292. Western blot analysis of CENP-F expression in IMR-32 nuclear extract.

Anti-CENPF antibody - ab84697 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab84697 staining CENPF in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour at 22°C. ab150081, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG H&L polyclonal (1/200) was used as the secondary antibody. Counterstained with DAPI.See Abreview

Anti-CENPF antibody - ab108483 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab108483 stained HeLa cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab108483, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% paraformaldehyde fixed (10 min) MCF7 and HepG2 cells at 5µg/ml, and in 100% methanol fixed (5 min) Hek293 cells at 5µg/ml.

Anti-CENPF antibody - ab198867 from Abcam

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling CENPF with ab198867 at 1/30.

Anti-CENPF antibody - ab93236 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human CENPF in Acetone fixed asynchronous HeLa cells by Immunocytochemistry using ab93236 at a dilution of 1/250. Detection: FITC conjugated anti-rabbit IgG used at a dilution of 1/100.

Anti-CENPF antibody - ab115281 from Abcam

Prices $376.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Human Kidney formalin-fixed, paraffin-embedded tissue stained with ab115281 at 3.75 ug/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-CENPF antibody - ab5 from Abcam

Prices $403.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
ab5 staining CENPF in Mouse embryonic fibroblast cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 3% BSA for 30 minutes at 23°C. Samples were incubated with primary antibody (1/300 in PBS + 0.1% Triton-X 100 + 1% BSA) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.See Abreview

Anti-CENPF antibody [14C10 /1D8] - ab90 from Abcam

Prices $391.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 14C10 /1D8
ab90 (1/200) staining CENPF in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.See Abreview

Anti-CENPF antibody [14C10 1D8] - ab118857 from Abcam

Prices $378.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 14C10 1D8
Human Liver (formalin-fixed, paraffin-embedded) stained with ab118857 at 10 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-CENPF / CENP-F Antibody (aa2350-2400) - LS-C286556 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human CENP-F/Mitosin by Immunohistochemistry. Sample: FFPE section of human Ewing sarcoma. Antibody: Affinity purified rabbit anti-CENP-F/Mitosin used at a dilution of 1:500.

Anti-CENPF / CENP-F Antibody (aa1759-2093) IHC-plus™ - LS-B3157 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Anti-CENPF antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3157 concentration 10 ug/ml.

Anti-CENPF / CENP-F Antibody (aa1759-2093, clone 14C10 1D8) IHC-plus™ - LS-B3046 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Anti-CENPF antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3046 concentration 10 ug/ml.

Anti-CENPF / CENP-F Antibody (aa1759-2093, clone 4H9) - LS-C53345 from LifeSpan Bioscience

Prices $445.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 4H9

Anti-CENPF / CENP-F Antibody - LS-C79060 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal

Anti-CENPF / CENP-F Antibody (aa1759-2093, clone 7F11.2) - LS-C20117 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 7F11.2

Anti-CENPF / CENP-F Antibody (C-Terminus, Biotin) - LS-C141539 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-CENPF / CENP-F Antibody (C-Terminus, DY488) - LS-C142236 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-CENPF / CENP-F Antibody (C-Terminus, DY650) - LS-C142235 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-CENPF / CENP-F Antibody (clone 2161C1a) - LS-C184374 from LifeSpan Bioscience

Prices $335.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 2161C1a

Anti-CENPF / CENP-F Antibody (C-Terminus, HRP) - LS-C142237 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-CENPF / CENP-F Antibody (C-Terminus, DY550) - LS-C142238 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-CENPF / CENP-F Antibody (aa3150-3200) - LS-C288402 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-CENPF / CENP-F Antibody (clone 2161C1a) - LS-C342838 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2161C1a
Western blot analysis of immunized recombinant protein, using anti-CENPF monoclonal antibody.
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