| Gene Symbol | CENPF |
|---|---|
| Entrez Gene | 1063 |
| Alt Symbol | CENF, PRO1779, hcp-1 |
| Species | Human |
| Gene Type | protein-coding |
| Description | centromere protein F, 350/400kDa |
| Other Description | AH antigen|CENP-F kinetochore protein|cell-cycle-dependent 350K nuclear protein|centromere protein F|centromere protein F, 350/400kDa (mitosin)|centromere protein F, 350/400ka (mitosin)|kinetochore protein CENPF|mitosin |
| Swissprots | Q5VVM7 Q13171 P49454 Q13246 |
| Accessions | CBX47616 EAW93350 P49454 AK307690 BC172232 AAI72232 BG680819 BQ774943 BU155312 BU662228 CD654009 U19769 AAA82889 U25725 AAA86889 U30872 AAA82935 XM_011509082 XP_011507384 XM_011509083 XP_011507385 NM_016343 NP_057427 |
| Function | Required for kinetochore function and chromosome segregation in mitosis. Required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1. Regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation. May play a regulatory or permissive role in the normal embryonic cardiomyocyte cell cycle and in promoting continued mitosis in transformed, abnormally dividing neonatal cardiomyocytes. Interaction with RB directs embryonic stem cells toward a cardiac lineage. Involved in the regulation of DNA synthesis and hence cell cycle progression, via its C-terminus. Has a potential role regulating skeletal myogenesis and in cell differentiation in embryogenesis. Involved in dendritic cell regulation of T-cell immunity ag |
| Subcellular Location | Cytoplasm, perinuclear region. Nucleus matrix. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Note=Relocalizes to the kinetochore/centromere (coronal surface of the outer plate) and the spindle during mitosis. Observed in nucleus during interphase but not in the nucleolus. At metaphase becomes localized to areas including kinetochore and mitotic apparatus as well as cytoplasm. By telophase, is concentrated within the intracellular bridge at either side of the mid-body. |
CENP-F CRISPR/Cas9 KO Plasmid (h) - sc-402178 from Santa Cruz Biotechnology
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CENP-F HDR Plasmid (h) - sc-402178-HDR from Santa Cruz Biotechnology
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CENP-F Double Nickase Plasmid (h) - sc-402178-NIC from Santa Cruz Biotechnology
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CENP-F Double Nickase Plasmid (h2) - sc-402178-NIC-2 from Santa Cruz Biotechnology
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CENP-F CRISPR Activation Plasmid (h) - sc-402178-ACT from Santa Cruz Biotechnology
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CENP-F CRISPR Activation Plasmid (h2) - sc-402178-ACT-2 from Santa Cruz Biotechnology
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CENP-F Lentiviral Activation Particles (h) - sc-402178-LAC from Santa Cruz Biotechnology
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CENP-F Lentiviral Activation Particles (h2) - sc-402178-LAC-2 from Santa Cruz Biotechnology
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CENP-F siRNA (h) - sc-37563 from Santa Cruz Biotechnology
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CENP-F shRNA Plasmid (h) - sc-37563-SH from Santa Cruz Biotechnology
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CENP-F shRNA (h) Lentiviral Particles - sc-37563-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU047311 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN5942 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND5942 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR06825 from Sigma-Aldrich
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CENPF siRNA (Human) - CRH0767 from Cohesion Biosciences
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CENPF - MBS8210755 from MyBioSource
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shRNA set against Human CENPF (NM_016343.3) - SHH261629 from Creative biogene
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shRNA set against Human CENPF(NM_016343.3) - SHG168073 from Creative biogene
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CENPF shRNA Plasmid (Human) - CHH0767 from Cohesion Biosciences
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CENPF miRNA 3'UTR clone - MiUTR1H-02038 from Creative biogene
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