Western blot analysis of extracts from NIH/3T3 cells, λ phosphatase- or PDGF-treated, using Non-phospho PTEN (Ser380/Thr382/Thr383) Antibody (upper) or PTEN (138G6) Rabbit mAb #9559 (lower). The non-phospho-specificity of the antibody was verified by preincubating the antibody with no peptide, with PTEN (Ser380/Thr382/Thr383) non-phosphopeptide or with PTEN (Ser380/Thr382/Thr383) phosphopeptide prior to incubating the membrane.