Western blot analysis of extracts from HeLa cells untransfected (left lane) and transfected by HA-SGK (right lane), using Phospho-SGK (Ser78) Antibody (upper) or control SGK Antibody #3272 (lower). The phospho-specificity of the antibody was characterized by treating the membrane with or without calf intestinal alkaline phosphatase (CIP) after Western transfer.