Immunoprecipitation of SHMT1 from extracts of HeLa cells using Normal Rabbit IgG #2729 (lane 2) or SHMT1 Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using SHMT1 Antibody. Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb #3677 and Anti-mouse IgG, HRP-linked Antibody #7076 were used as secondary antibodies.