Figure 1: IRAK4 protein from KARPAS-299 cells, serum-starved overnight and either left untreated (-) or treated with Human Interleukin-1β (hIL-1β) #8900 (50 ng/ml, 15 min; +), was detected using the PathScan® Total IRAK4 Sandwich ELISA Kit #13336. The absorbance readings at 450 nm are shown in the top figure while the corresponding western blot using IRAK4 Antibody #4363 is shown in the bottom figure.