The relationship between the protein concentration of the lysate from untreated and insulin-treated hSkMC cells and the absorbance at 450 nm using PathScan® Phospho-IRS-1 (Ser302) Sandwich ELISA Antibody Pair #7284 is shown. After overnight starvation, hSkMC cells were treated with insulin (100 nM) for 15 minutes at 37ºC and then lysed.