Figure 1. The relationship between the protein concentration of the lysate from untreated and anti-CD3-treated Jurkat cells and the absorbance at 450 nm using the PathScan® Phospho-LAT (Tyr191) Sandwich ELISA Antibody Pair #7937 is shown. After a 48 hour starvation, Jurkat cells were treated with anti-CD3 (10 µg/ml) for 2 minutes at 37ºC and then lysed.