Immunoprecipitation of VCAM-1 from HUVEC extracts, treated with Human Tumor Necrosis Factor-α (hTNF-α) #8902 (10 ng/ml, 16 hr), using Normal Rabbit IgG #2729 (lane 2) or VCAM-1 Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using VCAM-1 Antibody.